Dr Joop van den Heuvel

Dr Joop van den Heuvel
Head of Research Group
+49 531 6181-7046

Dr Joop van den Heuvel

Joop van den Heuvel studied biochemistry at the Free University in Amsterdam and also passed his PhD there from 1985 to 1990 about the effect of mRNA structure on the translational efficiency.

After that, he started as a postdoc at the former German Research Centre for Biotechnology (GBF) in Braunschweig, where he worked on the production and purification of proteins. From then on, Joop van den Heuvel has been faithful to this research field. In 1996, he became a project leader in the Department of Biochemical Engineering, and in 2002 he started to work in the Department of Structural Biology.

Since 2007, he leads the “Protein Sample Production Facility“ (PSPF) of the HZI, which uses mammalian cells for a large-scale production of recombinant proteins. One year later, he additionally became the leader of the research group “Recombinant Protein Expression”, where he and his colleagues are involved in research as well as in commissioned work.

Publications
Schütz A., Bernhard F., Berrow N., Buyel J.F., Ferreira-da-Silva F., Haustraete J., van den Heuvel J., Hoffmann J.E., de Marco A., Peleg Y., Suppmann S., Unger T., Vanhoucke M., ... , Witt S., Remans K. (2023)
A concise guide to choosing suitable gene expression systems for recombinant protein production
STAR.Protoc., 4 (4)
DOI: 10.1016/j.xpro.2023.102572 HZI-Repositorium PubMed
Katzmarzyk M., Clesle D.C., van den Heuvel J., Hoffmann M., Garritsen H., Pöhlmann S., ... , Jacobsen H., Cicin-Sain L. (2023)
Systematical assessment of the impact of single spike mutations of SARS-CoV-2 Omicron sub-variants on the neutralization capacity of post-vaccination sera
Front.Immunol., 14
DOI: 10.3389/fimmu.2023.1288794 HZI-Repositorium PubMed
Schwanke H., Goncalves Magalhäes V, Schmelz S., Wyler E., Hennig T., Günther T., Grundhoff A., Dölken L., Landthaler M., Van Ham M., Jänsch L., Büssow K., van den Heuvel J., Blankenfeldt W., Friedel C.C., ... , Erhard F., Brinkmann M.M. (2023)
The Cytomegalovirus M35 Protein Directly Binds to the Interferon-b[beta] Enhancer and Modulates Transcription of Ifnb1 and Other IRF3-Driven Genes
J.Virol., 97 (6)
DOI: 10.1128/jvi.00400-23 HZI-Repositorium PubMed
Kaipa J.M., Krasnoselska G., Owens R.J., van den Heuvel J. (2023)
Screening of Membrane Protein Production by Comparison of Transient Expression in Insect and Mammalian Cells
Biomolecules., 13 (5)
DOI: 10.3390/biom13050817 HZI-Repositorium PubMed
Sun S., Fushimi M., Rossetti T., Kaur N., Ferreira J., Miller M., Quast J., van den Heuvel J., Steegborn C., Levin L.R., Buck J., Myers R.W., Kargman S., Liverton N., ... , Meinke P.T., Huggins D.J. (2023)
Scaffold Hopping and Optimization of Small Molecule Soluble Adenyl Cyclase Inhibitors Led by Free Energy Perturbation
J.Chem.Inf.Model., 63 (9)
DOI: 10.1021/acs.jcim.2c0157 PubMed
Miller M., Rossetti T., Ferreira J., Ghanem L., Balbach M., Kaur N., Levin L.R., Buck J., Kehr M., Coquille S., van den Heuvel J., Steegborn C., Fushimi M., Finkin-Groner E., Myers R.W., Kargman S., Liverton N.J., ... , Huggins D.J., Meinke P.T. (2022)
Design, Synthesis, and Pharmacological Evaluation of Second-Generation Soluble Adenylyl Cyclase (sAC, ADCY10) Inhibitors with Slow Dissociation Rates
J.Med.Chem.
DOI: 10.1021/acs.jmedchem.2c01133 HZI-Repositorium PubMed
Jaron M., Lehky M., Zarà M., Zaydowicz C.N., Lak A., Ballmann R., Heine P.A., Wenzel E.V., Schneider K.T., Bertoglio F., Kempter S., Köster R.W., Barbieri S.S., van den Heuvel J., Hust M., ... , Dübel S., Schubert M. (2022)
Baculovirus-Free SARS-CoV-2 Virus-like Particle Production in Insect Cells for Rapid Neutralization Assessment
Viruses, 14 (10)
DOI: 10.3390/v14102087 HZI-Repositorium PubMed
Reboll M.R., Klede S., Taft M.H., Cai C.-L., Field L.J., Lavine K.J., Koenig A.L., Fleischauer J., Meyer J., Schambach A., Niessen H.W., Kosanke M., van den Heuvel J., Pich A., Bauersachs J., Wu X., Zheng L., Wang Y., Korf-Klingebiel M., ... , Polten F., Wollert K.C. (2022)
Meteorin-like promotes heart repair through endothelial KIT receptor tyrosine kinase
Science, 376 (6599)
DOI: 10.1126/science.abn3027 HZI-Repositorium PubMed
Sevenich M., van den Heuvel J., Gering I., Mohrlüder J., Willbold D. (2022)
A So-Far Overlooked Secondary Conformation State in the Binding Mode of SARS-CoV-2 Spike Protein to Human ACE2 and Its Conversion Rate Are Crucial for Estimating Infectivity Efficacy of the Underlying Virus Variant
J.Virol.
DOI: 10.1128/jvi.00685-22 HZI-Repositorium PubMed
Peukert C., Popat Gholap S., Green O., Pinkert L., van den Heuvel J., Van Ham M., ... , Shabat D., Brönstrup M. (2022)
Enzyme-Activated, Chemiluminescent Siderophore-Dioxetane Probes Enable the Selective and Highly Sensitive Detection of Bacterial Pathogens
Angew.Chem.Int.Ed Engl.
DOI: 10.1002/anie.202201423 HZI-Repositorium PubMed
Sevenich M., Thul E., Lakomek N.A., Klünemann T., Schubert M., Bertoglio F., van den Heuvel J., Petzsch P., ... , Mohrlüder J., Willbold D. (2022)
Phage Display-Derived Compounds Displace hACE2 from Its Complex with SARS-CoV-2 Spike Protein
Biomedicines, 10 (2)
DOI: 10.3390/biomedicines10020441 HZI-Repositorium PubMed
Korf-Klingebiel M., Reboll M.R., Polten F., Weber N., Jäckle F., Wu X., Kallikourdis M., Kunderfranco P., Condorelli G., Giannitsis E., Kustikova O.S.., Schambach A., Pich A., Widder J.D., Bauersachs J., van den Heuvel J., Kraft T., ... , Wang Y., Wollert K.C. (2021)
Myeloid-Derived Growth Factor Protects Against Pressure Overload-Induced Heart Failure by Preserving Sarco/Endoplasmic Reticulum Ca(2+)-ATPase Expression in Cardiomyocytes
Circulation
DOI: 10.1161/CIRCULATIONAHA.120.053365 HZI-Repositorium PubMed
Bertoglio F., Fühner V., Ruschig M., Heine P.A., Abassi L., Klünemann T., Rand U., Meier D., Langreder N., Steinke S., Ballmann R., Schneider K., Roth K.D.R., Kuhn P., Riese P., Schäckermann D., Korn J., Koch A., Chaudhry M.Z., Eschke K., Kim Y., Zock-Emmenthal S., Becker M., Scholz M., Moreira G.M.S.G., Wenzel E.V., Russo G., Garritsen H.S.P., Casu S., Gerstner A., Roth G., Adler J., Trimpert J., Hermann A., Schirrmann T., Dübel Stefan, Frenzel A., van den Heuvel J., Cicin-Sain L., ... , Schubert M., Hust M. (2021)
A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations
Cell Rep., 36 (4)
DOI: 10.1016/j.celrep.2021 HZI-Repositorium PubMed
Fushimi M., Buck H., Balbach M., Gorovyy A., Ferreira J., Rossetti T., Kaur N., Levin L.R., Buck J., Quast J., van den Heuvel J., Steegborn C., Finkin-Groner E., Kargman S., Michino M., Foley M.A., Miller M., Liverton N.J., ... , Huggins D.J., Meinke P.T. (2021)
Discovery of TDI-10229: A Potent and Orally Bioavailable Inhibitor of Soluble Adenylyl Cyclase (sAC, ADCY10)
ACS Med.Chem.Lett.
DOI: 10.1021/acsmedchemlett.1c00273 HZI-Repositorium PubMed
Schubert M., Nimtz M., Bertoglio F., Schmelz S., Lukat P., van den Heuvel J. (2021)
Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells
, 3 (6)
DOI: 10.1007/978-1-0716-1406-8_6 HZI-Repositorium PubMed
König J., Hust M., van den Heuvel J. (2021)
Validation of the Production of Antibodies in Different Formats in the HEK 293 Transient Gene Expression System
Methods Mol.Biol, 2247 (4)
DOI: 10.1007/978-1-0716-1126-5_4 PubMed
Korn J., Schäckermann D., Kirmann T., Bertoglio F., Steinke S., Heisig J., Ruschig M., Rojas G., Langreder N., Wenzel E.V., Roth K.D.R., Becker M., Meier D., van den Heuvel J., Hust M., ... , Dübel S., Schubert M. (2020)
Baculovirus-free insect cell expression system for high yield antibody and antigen production
Sci.Rep., 10 (1)
DOI: 10.1038/s41598-020-78425-9 HZI-Repositorium PubMed
Testolin G., Cirnski K., Rox K., Prochnow H., Fetz V., Grandclaudon C., Mollner T., Baiyoumy A., Ritter A., Leitner C., Krull J., van den Heuvel J., Vassort A., Sordello S., Hamed M.M., Elgaher W.A.M., Herrmann J., Hartmann R.W., ... , Müller R., Brönstrup M. (2020)
Synthetic studies of cystobactamids as antibiotics and bacterial imaging carriers lead to compounds with high: In vivo efficacy
Chem.Sci., 11 (5)
DOI: 10.1039/c9sc04769g HZI-Repositorium
Bleckmann M., Schürig M., Endres M., Samuels A., Gebauer D., ... , Konisch N., van den Heuvel J. (2019)
Identifying parameters to improve the reproducibility of transient gene expression in High Five cells
PLoS ONE, 14 (6)
DOI: 10.1371/journal.pone.0217878 HZI-Repositorium PubMed
Büssow K., Themann P., Luu S., Pentrowski P., Harting C., Majewski M., Vollmer V., Köster M., Grashoff M., Zawatzky R., van den Heuvel J., ... , Kröger A., Böldicke T. (2019)
ER intrabody-mediated inhibition of interferon a secretion by mouse macrophages and dendritic cells
PLoS ONE, 14 (4)
DOI: 10.1371/journal.pone.0215062 HZI-Repositorium PubMed
Stolt-Bergner P., Benda C., Bergbrede T., Besir H., Celie P.H.N., Chang C., Drechsel D., Fischer A., Geerlof A., Giabbai B., van den Heuvel J., Huber G., Knecht W., Lehner A., Lemaitre R., Pardee G., Racke I., Remans K., Sander A., Scholz J., Stadnik M., Storici P., Weinbruch D., ... , Zaror I., Lua L.H.L. (2018)
Baculovirus-driven protein expression in insect cells: A benchmarking study
J.Struct.Biol., 203 (2)
DOI: 10.1016/j.jsb.2018.03.004 HZI-Repositorium PubMed
Marschall A.L.J., Single F.N., Schlarmann K., Bosio A., Strebe N., van den Heuvel J., ... , Frenzel A., Dübel S. (2014)
Functional knock down of VCAM1 in mice mediated by endoplasmatic reticulum retained intrabodies
MAbs, 6 (6)
Kaba Hani E.J., Maier N., Schliebe-Ohler N., Mayer Y., Müller Peter P., van den Heuvel Joop, Schuchhardt J., ... , Hanack K., Bilitewski Ursula (2015)
Identification of whole pathogenic cells by monoclonal antibodies generated against a specific peptide from an immunogenic cell wall protein
Journal of Microbiological Methods, 108
Kleinboelting S., van den Heuvel Joop, Steegborn C. (2014)
Structural analysis of human soluble adenylyl cyclase and crystal structures of its nucleotide complexes - Implications for cyclase catalysis and evolution
FEBS Journal, 281 (18)
Kleinboelting S., Diaz A., Moniot S., van den Heuvel Joop, Weyand M., Levin L.R., ... , Buck J., Steegborn C. (2014)
Crystal structures of human soluble adenylyl cyclase reveal mechanisms of catalysis and of its activation through bicarbonate
Proceedings of the National Academy of Sciences of the United States of America, 111 (10)
Buschart A., Gremmer K., El-Mowafy M., van den Heuvel Joop, Müller Peter P., Bilitewski Ursula (2012)
A novel functional assay for fungal histidine kinases group III reveals the role of HAMP domains for fungicide sensitivity
Journal of Biotechnology, 157 (1)
Meyer S., Lorenz C., Baser B., Wordehoff M., Jäger Volker, van den Heuvel Joop (2013)
Multi-host expression system for recombinant production of challenging proteins
PLoS ONE, 8 (7)
DOI: 10.1371/journal.pone.0068674 HZI-Repositorium PubMed
Ringel P., Krausze Joern, van den Heuvel Joop, Curth U., Pierik A.J., Herzog S., ... , Mendel R.R., Kruse T. (2013)
Biochemical characterization of molybdenum cofactor-free nitrate reductase from Neurospora crassa
Journal of Biological Chemistry, 288 (20)
Hofmeyer T., Schmelz S., Degiacomi M.T., Dal Peraro M., Daneschdar M., Scrima Andrea, van den Heuvel Joop, ... , Heinz Dirk W., Kolmar H. (2013)
Arranged sevenfold: Structural insights into the C-terminal oligomerization domain of human C4b-binding protein
Journal of Molecular Biology, 425 (8)
DOI: 10.1016/j.jmb.2012.12.017 HZI-Repositorium PubMed
Kugler J., Schmelz S., Gentzsch J., Haid S., Pollmann E., van den Heuvel Joop, Franke R., Pietschmann Thomas, ... , Heinz Dirk W., Collins J. (2012)
High Affinity Peptide Inhibitors of the Hepatitis C Virus NS3-4A Protease Refractory to Common Resistant Mutants
Journal of Biological Chemistry, 287 (46)
DOI: 10.1074/jbc.M112.393843 HZI-Repositorium PubMed
Iphofer A., Kummer A., Nimtz Manfred, Ritter A., Arnold T., Frank R., van den Heuvel Joop, Kessler B.M., ... , Jänsch Lothar, Franke R. (2012)
Profiling ubiquitin linkage specificities of deubiquitinating enzymes with branched ubiquitin isopeptide probes
ChemBioChem, 13 (10)
Wiesand U., Sorg I., Amstutz M., Wagner S., van den Heuvel Joop*, Lührs Thorsten*, ... , Cornelis G.R., Heinz Dirk W.* (2009)
Structure of the type III secretion recognition protein YscU from Yersinia enterocolitica
Journal of Molecular Biology, 385 (3)
DOI: 10.1016/j.jmb.2008.10.034 HZI-Repositorium PubMed
Niemann H.N., Jäger Volker*, Butler P.J.G., van den Heuvel Joop*, Schmidt S., Ferraris D., ... , Gherardi E., Heinz Dirk W.* (2007)
Structure of the human receptor tyrosine kinase met in complex with the Listeria monocytogenes
Cell, 130 (2)
DOI: 10.1016/j.cell.2007.05.037 HZI-Repositorium PubMed
Wollert T., Pasche Bastian*, Rochon Maike*, Deppenmeier S., van den Heuvel Joop*, Gruber A.D., Heinz Dirk W.*, ... , Lengeling Andreas*, Schubert Wolf-Dieter* (2007)
Extending the host range of Listeria monocytogenes
Cell, 129 (5)
Zieseniss A., Schroeder U., Buchmeier S., Schoenberger C., van den Heuvel Joop*, ... , Jockusch B., Illenberger S. (2007)
Raver1 is an integral component of muscle contractile elements
Cell and Tissue Research, 327 (3)
DOI: 10.1007/s00441-006-0322-1 HZI-Repositorium PubMed
Zheng P, Sun Jibin*, van den Heuvel Joop*, Zeng An-Ping* (2006)
Discovery and investigation of a new, second triose phosphate isomerase in Klebsiella pneumoniae
Journal of Biotechnology, 125 (4)
Zheng P., Wereath K., Sun Jibin*, van den Heuvel Joop*, Zeng An-Ping* (2006)
Overexpression of genes of the dha regulon and its effects on cell growth, glycerol fermentation to 1,3-propanediol and plasmid stability in Klebsiella pneumoniae
Process Biochemistry, 41 (10)
Dresler K., van den Heuvel Joop*, Müller Rolf-Joachim*, Deckwer Wolf-Dieter* (2006)
Production of a recombinant polyester-cleaving hydrolase from Thermobifida fusca
Bioprocess and Biosystems Engineering, 29
Kleeberg I., Welzel K., van den Heuvel J., Müller R.-J., Deckwer W.-D. (2005)
Characterization of a new extracellular hydrolase from Thermobifida fusca degrading aliphatic-aromatic copolyesters
Biomacromolecules, 6
Astner I., Schulze J.O., van den Heuvel J., Jahn D., Schubert W.-D., Heinz D.W. (2005)
Crystal structure of 5-aminolevulinate synthase, the first enzyme of heme biosynthesis, and its link to XLSA in humans
Embo Journal, 24
DOI: 10.1038/sj.emboj.7600792 HZI-Repositorium
Bollati-Fogolin M., Irani N., Beccaria A.J., Schulz C., van den Heuvel J., Elias C.B., Carpentier E., Durocher Y., Bisson L., Etcheverrigaray M., Kratje R.B., Wirth M., ... , Kamen A., Wagner R. (2005)
Impact of yeast pyruvate carboxylase on the productivity of animal host cell lines
Hoffmann F., van den Heuvel J., Zidek N., Rinas U. (2004)
Minimizing inclusion body formation during recombiant protein production in Escherichia coli at bench and pilot plant scale.
Enzyme and Microbial Technology, 34
Ross A., Kessler W., Krumme D., Menge U., Wissing J., ... , van den Heuvel J., Flohé L. (2004)
Optimised fermentation strategy for 13C/15N recombinant protein labelling in Escherichia coli for NMR-structure analysis
Journal of Biotechnology, 108
Sun J., van den Heuvel J., Soucaille P., Qu Y., Zeng A.P. (2003)
Comparative genomic analysis of dha regulon and related genes for anaerobic glycerol metabolism in microorganisms
Biotechnology progress, 19
Pipp F., Heil M., Issbrücker K., Ziegelhoeffer T., Martin S., van den Heuvel J., Weich H., Fernandez B., Colomb G., Caremeliet P., ... , Schaper W., Claus M. (2003)
The VEGFR-1 selective VEGF-homologue PIGF is arteriogenic: evidence for a monocyte mediated mechanism.
Circulation Research, 92
Heil M., Mittnacht-Krauss R., Issbrücker K., van den Heuvel J., Dehio C., Schaper W., ... , Clauss M., Weich H.A. (2003)
An engineered heparin-binding form of VEGF-E: biological effects in vitro and mobilisation of precursor cells.
Angiogenesis, 6 (3)
van den Heuvel J., Heinz D.W. (2002)
"Plug and Play"-expression systems for high-quality production of recombinant proteins for structural analysis
Gene Function & Disease, 3 (1/2)
Henze B., Bilitewski U., van den Heuvel J. (2002)
Biosensorsysteme zur Genexpressionsanalytik
Henze B., Bebber C., van den Heuvel J., Bilitewski U. (2002)
Detection of mRNA using the BIAcore
Gouda M.K., Kleeberg I., van den Heuvel J., Müller R.-J., Deckwer W.-D. (2002)
Production of a polyester degrading extracellular hydrolase from Thermomonspora fusca
Biotechnology progress, 18
Sun J., van den Heuvel J., Zeng A.-P. (2001)
Reconstruction of metabolic and regulatory networks of Klebsiella pneumoniae from genome data

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