Our service
Customized histo- pathology work for your research:
- Blinded and randomized histo-pathological evaluation (scores) specifically designed for your research question inclusive the interpretation and illustration of your data (approx. 50 projects per year)
- More than 100 antigen specific staining methods for bright field and fluorescence microscopy as single or double staining
- We establish staining methods upon your demand.
- Animal and human tissues as well as cytological samples and even cell cultures can be processed.
Overview of our technical services:
- Fixation using Formaldehyde, Ethanol-Carnoy, HOPE
- Tissue embedding and sectioning in Paraffin, cryo and plastic
- Automated hematoxylin-eosin (HE)- staining
- Histo-chemical staining (i.e. PAS, Masson-Trichrom, Ziehl-Neeslen, Gram staining, …)
- Immun-histo-chemical staining (i.e. CD4, FoxP3, CD11c, Influenza A, MCMV, …)
- Every antibody staining is possible in fluorescence as well
- Double staining (i.e. CD4/Caspase3, CD3/FoxP3), fluorescence and histo-chemical
Our service
Customized histo- pathology work for your research:
- Blinded and randomized histo-pathological evaluation (scores) specifically designed for your research question inclusive the interpretation and illustration of your data (approx. 50 projects per year)
- More than 100 antigen specific staining methods for bright field and fluorescence microscopy as single or double staining
- We establish staining methods upon your demand.
- Animal and human tissues as well as cytological samples and even cell cultures can be processed.
Overview of our technical services:
- Fixation using Formaldehyde, Ethanol-Carnoy, HOPE
- Tissue embedding and sectioning in Paraffin, cryo and plastic
- Automated hematoxylin-eosin (HE)- staining
- Histo-chemical staining (i.e. PAS, Masson-Trichrom, Ziehl-Neeslen, Gram staining, …)
- Immun-histo-chemical staining (i.e. CD4, FoxP3, CD11c, Influenza A, MCMV, …)
- Every antibody staining is possible in fluorescence as well
- Double staining (i.e. CD4/Caspase3, CD3/FoxP3), fluorescence and histo-chemical
Dr Marina Greweling-Pils
Mouse pathology can give us an overview – offer a first glimpse, if you will – of the relationship between the infection and the immune response.
Marina Greweling-Pils studied veterinary medicine at the Hanover Veterinary School and the École Nationale Veterinaire de Lyon (France). After her graduation in 2004, she came to the Helmholtz Centre for her PhD thesis. Her research at the HZI focused on colitis and infections. During her Postdoc at the Hanover Medical School, Dr Greweling-Pils worked on the induction of tolerance in organ transplantation. She returned to the HZI as deputy head of the animal facility in 2009 and since 2011, she is head of the mouse pathology platform.
Team
Selected Publications
Osbelt, L., Almási, É. D. H., Wende, M., Kienesberger, S., Voltz, A., Lesker, T. R., Muthukumarasamy, U., Knischewski, N., Nordmann, E., Bielecka, A. A., Giralt-Zúñiga, M., Kaganovitch, E., Kühne, C., Baier, C., Pietsch, M., Müsken, M., Greweling-Pils, M. C., Breinbauer, R., Flieger, A., Schlüter, D., … Strowig, T. (2024). Klebsiella oxytoca inhibits Salmonella infection through multiple microbiota-context-dependent mechanisms. Nature Microbiology, 9(7), 1792–1811. DOI: 10.1038/s41564-024-01710-0
Rox, K., Jansen, R., Lukežič, T., Greweling-Pils, M., Herrmann, J., Miethke, M., Hüttel, S., Hennessen, F., Abou Fayad, A., Holzhausen, C., Lundberg, C. V., Teague, J., Sudarman, E., Bülter, L., Hesterkamp, T., Stadler, M., Brönstrup, M., & Müller, R. (2024). Pharmacokinetic and pharmacodynamic evaluation of the atypical tetracyclines chelocardin and amidochelocardin in murine infection models. Microbiology spectrum, 12(1), e0128923. DOI: 10.1128/spectrum.01289-23
Abassi, L., Bertoglio, F., Mačak Šafranko, Ž., Schirrmann, T., Greweling-Pils, M., Seifert, O., Khan, F., Katzmarzyk, M., Jacobsen, H., Gödecke, N., Heine, P. A., Frenzel, A., Nowack, H., Dübel, S., Kurolt, I. C., Kontermann, R. E., Markotić, A., Schubert, M., Hust, M., & Čičin-Šain, L. (2023). Evaluation of the Neutralizing Antibody STE90-C11 against SARS-CoV-2 Delta Infection and Its Recognition of Other Variants of Concerns. Viruses, 15(11), 2153. DOI: 10.3390/v15112153
Metzdorf, K., Jacobsen, H., Greweling-Pils, M. C., Hoffmann, M., Lüddecke, T., Miller, F., Melcher, L., Kempf, A. M., Nehlmeier, I., Bruder, D., Widera, M., Ciesek, S., Pöhlmann, S., & Čičin-Šain, L. (2023). TMPRSS2 Is Essential for SARS-CoV-2 Beta and Omicron Infection. Viruses, 15(2), 271. DOI: 10.3390/v15020271
Herppich, S., Hoenicke, L., Kern, F., Kruse, F., Smout, J., Greweling-Pils, M. C., Geffers, R., Burton, O. T., Liston, A., Keller, A., Floess, S., & Huehn, J. (2023). Zfp362 potentiates murine colonic inflammation by constraining Treg cell function rather than promoting Th17 cell differentiation. European journal of immunology, 53(10), e2250270. DOI: 10.1002/eji.202250270
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