© AdobeStock/Artur
Unsere Forschung
Proteine sind die funktionellen Bausteine in Infektionen und Immunität sowie bevorzugte Ziele der Wirkstoffforschung. Unsere Forschung konzentriert sich auf die Charakterisierung der Reaktionen primärer Immunzellen durch Proteomanalysen. Immunzellen nutzen unterschiedliche Protein-Netzwerke, um den Schutz gegen Pathogene zu vermitteln. Hierfür bilden Immunzellen aktivierende und/oder sekretorische Immunsynapsen (IS). Diese Synapsen organisieren alle Zellkontakt-abhängigen Antworten einschließlich Antigenerkennung und Zytokinfreisetzung.
Unsere Forschung soll zum Verständnis beitragen, wie Proteinnetzwerke an Synapsen die Aktivität von Immunzellen kontrollieren (NK-, MAIT- und Treg-Zellen). Projekte sollen insbesondere neue immunregulatorische Mechanismen mit translationaler und klinischer Bedeutung aufklären.
Für die Phänotypisierung von Immunzellen kombinieren wir moderne Technologien der Proteomanalyse mit Verfahren der molekularen Immunologie und Zellbiologie. Proteinnetzwerke an Synapsen werden hauptsächlich durch post-translationale Protein-Modifizierungen kontrolliert (Phosphorylierung, Ubiquitinierung, Vesikeltransport, ROS/Metaboliten), die wir direkt durch Massenspektrometrie bestimmen können.
Zentrale Aspekte unserer Forschung sind:
- Analyse der in vitro & in vivo Immunantwort primärer Zellen durch Proteomik.
- Identifizierung regulatorischer Mechanismen an immunologischen Synapsen.
- Mikrobiom-basierte Immunität von Mukosa-assoziierten invarianten T Zellen (MAITs).
- Individuelle Phänotypisierung von Immunzellen während Infektionen und Therapie.
Unsere Forschung
Proteine sind die funktionellen Bausteine in Infektionen und Immunität sowie bevorzugte Ziele der Wirkstoffforschung. Unsere Forschung konzentriert sich auf die Charakterisierung der Reaktionen primärer Immunzellen durch Proteomanalysen. Immunzellen nutzen unterschiedliche Protein-Netzwerke, um den Schutz gegen Pathogene zu vermitteln. Hierfür bilden Immunzellen aktivierende und/oder sekretorische Immunsynapsen (IS). Diese Synapsen organisieren alle Zellkontakt-abhängigen Antworten einschließlich Antigenerkennung und Zytokinfreisetzung.
Unsere Forschung soll zum Verständnis beitragen, wie Proteinnetzwerke an Synapsen die Aktivität von Immunzellen kontrollieren (NK-, MAIT- und Treg-Zellen). Projekte sollen insbesondere neue immunregulatorische Mechanismen mit translationaler und klinischer Bedeutung aufklären.
Für die Phänotypisierung von Immunzellen kombinieren wir moderne Technologien der Proteomanalyse mit Verfahren der molekularen Immunologie und Zellbiologie. Proteinnetzwerke an Synapsen werden hauptsächlich durch post-translationale Protein-Modifizierungen kontrolliert (Phosphorylierung, Ubiquitinierung, Vesikeltransport, ROS/Metaboliten), die wir direkt durch Massenspektrometrie bestimmen können.
Zentrale Aspekte unserer Forschung sind:
- Analyse der in vitro & in vivo Immunantwort primärer Zellen durch Proteomik.
- Identifizierung regulatorischer Mechanismen an immunologischen Synapsen.
- Mikrobiom-basierte Immunität von Mukosa-assoziierten invarianten T Zellen (MAITs).
- Individuelle Phänotypisierung von Immunzellen während Infektionen und Therapie.
Prof. Lothar Jänsch
Proteine sind die funktionellen Einheiten des Lebens. Erst wenn wir sie in ihrer Gesamtheit verstehen, dann wissen wir, wie das Leben funktioniert!
© HZI/Janos Krüger
Lothar Jänsch hat an der Freien Universität Berlin Biologie studiert und 1995 seine Diplomarbeit am Institut für Genbiologische Forschung (IGF) in Berlin angefertigt. Anschließend arbeitete er als Doktorand am IGF und an der Universität Hannover und schloss 1998 mit der Promotion ab.
Nach einer zweijährigen Tätigkeit als wissenschaftlicher Mitarbeiter bei der Firma Pfizer wechselte Lothar Jänsch im Jahr 2000 als Postdoc in die Abteilung Zellbiologie der damaligen Gesellschaft für Biotechnologische Forschung (GBF). Von 2003 bis 2006 leitete er dort das Projekt „Identifikation neuer Virulenzfaktoren“ und übernahm 2009 die Leitung der Arbeitsgruppe „Zelluläre Proteomforschung“ am Helmholtz-Zentrum für Infektionsforschung (HZI). Gemeinsam mit der Technischen Universität Braunschweig wurde er 2011 auf die W2 Professur „Proteomforschung in Infektionsprozessen“ berufen.
Sein Forschungsinteresse bilden translationale Projekte mit akademischen, medizinischen und industriellen Partnern, in denen Pathogen-Wirts-Interaktome aufgeklärt und die Kontrollmechanismen von Immunantworten charakterisiert werden.
Team
Prof. Dr. Lothar Jänsch
Forschungsgruppenleiter
Zelluläre Proteomforschung |
Proteomanalytik
HZI Campus
Prof. Dr. Frank Klawonn
Projektleiter
Zelluläre Proteomforschung
HZI Campus
Dr. Marco van Ham
Wissenschaftler
Zelluläre Proteomforschung
HZI Campus
Dr. Dominik Körner
Postdoc
Zelluläre Proteomforschung
HZI Campus
M.Sc. Jenny Kronemann
Doktorandin
Zelluläre Proteomforschung
HZI Campus
Qile Yang
Doktorand
Zelluläre Proteomforschung
HZI Campus
Tina Rietschel
Doktorandin
Zelluläre Proteomforschung
HZI Campus
Undine Felgenträger
Technische Assistentin
Zelluläre Proteomforschung
HZI Campus
Reiner Munder
Technischer Assistent
Zelluläre Proteomforschung
HZI Campus
Kirsten Minkhart
Technische Assistentin
Zelluläre Proteomforschung
HZI Campus
Anja Meier
Technische Assistentin
Zelluläre Proteomforschung
HZI Campus
Andrea Abrahamik
Technische Assistentin
Zelluläre Proteomforschung
HZI Campus
Hedwig Schrader
Technische Assistentin
Zelluläre Proteomforschung
HZI Campus
Amani Al Mekhlafi
Doktorandin
Zelluläre Proteomforschung
HZI Campus
Dr. Christian Erck
Wissenschaftler
Zelluläre Proteomforschung
HZI Campus
Lena Seela
Technische Assistentin
Zelluläre Proteomforschung
HZI Campus
Anna Duwensee
Technische Assistentin
Zelluläre Proteomforschung
HZI Campus
Dunja König
Sekretärin
Zelluläre Proteomforschung
HZI Campus
Ausgewählte Publikationen
Bernal I, Hofmann JD, Bulitta B, Klawonn F, Michel AM, Jahn D, Neumann-Schaal M, Bruder D, Jänsch L (2018) Clostridioides difficile activates human mucosal-associated invariant T cells. Front Microbiol Volume: 9: 2532
van Ham M, Teich R, Philipsen L, Niemz J, Amsberg N, Wissing J, Nimtz M, Gröbe L, Kliche S, Thiel N, Klawonn F, Hubo M, Jonuleit H, Reichardt P, Müller AJ, Huehn J, Jänsch L (2017) TCR signalling network organization at the immunological synapses of murine regulatory T cells. Eur J Immunol 47: 2043–58
Lingel H, Wissing J, Arra A, Schanze D, Lienenklaus S, Klawonn F, Pierau M, Zenker M, Jansch L*, Brunner-Weinzierl* MC (2017) CTLA-4-mediated posttranslational modifications direct cytotoxic T-lymphocyte differentiation. Cell Death & Differ 24: 1739–49 * correspondence
Shevchuk O, Abidi N, Klawonn F, Wissing J, Nimtz M, Kugler C, Steinert M, Goldmann T, Jänsch L (2014) HOPE-fixation of lung tissue allows retrospective proteome and phosphoproteome studies. J Proteome Res 13: 5230-9
Scheiter M, Lau U, Van Ham M, Bulitta B, Grobe L, Garritsen H, Klawonn F, Konig S, Jänsch L (2013) Proteome analysis of distinct developmental stages of human natural killer (NK) cells. Mol Cell Proteomics 12: 1099-14
Publikationen
Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization
Microorganisms.,
12
(10)
Long-Term Human Immune Reconstitution, T-Cell Development, and Immune Reactivity in Mice Lacking the Murine Major Histocompatibility Complex: Validation with Cellular and Gene Expression Profiles
Cells,
13
(20)
Proteomic analysis of plasma-derived extracellular vesicles: pre- and postprandial comparisons
Sci.Rep.,
14
(1)
Correction to: Results of a monocentric field study: value of histology compared to sonication method and conventional tissue culture in the diagnosis of periprosthetic joint infection (PJI) (Infection, (2024), 10.1007/s15010-024-02278-x)
Infection
Peptide-Bound Glycative, AGE and Oxidative Modifications as Biomarkers for the Diagnosis of Alzheimer's Disease-A Feasibility Study
Biomedicines.,
12
(9)
Patient-Oriented Questionnaires and Machine Learning for Rare Disease Diagnosis: A Systematic Review
J.Clin.Med.,
13
(17)
At the crossroads between statistics and artificial intelligence: statistical learning in laboratory medicine
J.Lab.Med.,
48
(5)
Bronchodilator Response in Post-COVID-19 Patients Undergoing Pulmonary Rehabilitation
Respiration
A Novel Tool for the Rapid and Transparent Verification of Reference Intervals in Clinical Laboratories
Journal of Clinical Medicine,
13
(15)
Impact of virtual reality on peri-interventional pain, anxiety and distress in a pediatric oncology outpatient clinic: a randomized controlled trial
BMC Pediatrics,
24
(1)
Standardization with zlog values improves exploratory data analysis and machine learning for laboratory data
Journal of Laboratory Medicine
Formation of the pyruvoyl-dependent proline reductase Prd from Clostridioides difficile requires the maturation enzyme PrdH
PNAS Nexus,
3
(7)
Clinical and Diagnostic Features of Post-Acute COVID-19 Vaccination Syndrome (PACVS)
Vaccines,
12
(7)
Targeted metabolomics identifies accurate CSF metabolite biomarkers for the differentiation between COVID-19 with neurological involvement and CNS infections with neurotropic viral pathogens
Journal of Translational Medicine,
22
(1)
Dynamic changes in the proximitome of neutral sphingomyelinase-2 (nSMase2) in TNFa[alpha] stimulated Jurkat cells
Frontiers in Immunology,
15
Cluster-Centered Visualization Techniques for Fuzzy Clustering Results to Judge Single Clusters
Appl.Sci.,
14
(3)
An Objective Function-Based Clustering Algorithm with a Closed-Form Solution and Application to Reference Interval Estimation in Laboratory Medicine
Algorithms,
17
(4)
Results of a monocentric field study: value of histology compared to sonication method and conventional tissue culture in the diagnosis of periprosthetic joint infection (PJI)
Infection
Parenting and Gender as Impact Factors for Social Participation, Quality of Life, and Mental Health in Long COVID
J.Prim.Care Community Health,
15
Evaluating the Feasibility, Acceptance, and Beneficial Effects of Online Occupational Therapy for Post-COVID-19 Condition: Protocol for a Randomized Controlled Trial (ErgoLoCo Study)
JMIR.Res.Protoc.,
13
Importance and Uncertainty of l[lambda]-Estimation for Box-Cox Transformations to Compute and Verify Reference Intervals in Laboratory Medicine
Stats,
7
(1)
Cluster analysis of long COVID symptoms for deciphering a syndrome and its long-term consequence
Immunologic Research
PATHFINDER-CHD: prospective registry on adults with congenital heart disease, abnormal ventricular function, and/or heart failure as a foundation for establishing rehabilitative, prehabilitative, preventive, and health-promoting measures: rationale, aims, design and methods
BMC Cardiovasc.Disord.,
24
(1)
The Role of Neutral Sphingomyelinase-2 (NSM2) in the Control of Neutral Lipid Storage in T Cells
Int.J.Mol.Sci,
25
(6)
Quantification of ventricular stress in univentricular hearts during early childhood using age-independent zlog-NT-proBNP
Int.J.Cardiol.
Activity-Based Protein Profiling Identifies Protein Disulfide-Isomerases as Target Proteins of the Volatile Salinilactones
Adv.Sci.
Association of long COVID with health-related Quality of Life and Social Participation in Germany: Finding from an online-based cross-sectional survey
Heliyon.,
10
(4)
Longitudinal analysis of systemic ventricular function and atrioventricular valve function after the norwood procedure
Eur.J.Cardiothorac.Surg.
Improvement of differential diagnosis of lung cancer by use of multiple protein tumor marker combinations
Tumour.Biol.
Optimal Selection of Sampling Points within Sewer Networks for Wastewater-Based Epidemiology Applications
Methods Protoc.,
7
(1)
Elevated phospholipids and acylcarnitines C4 and C5 in cerebrospinal fluid distinguish viral CNS infections from autoimmune neuroinflammation
J.Transl.Med.,
21
(1)
Impact of pulsatile pulmonary blood flow on cardiopulmonary exercise performance after the Fontan procedure
JTCVS Open
Sex-specific differences in pain localization in female patients with endometriosis: A comparison of sexless and female human body outlines
Brain Behav.
Method Comparison and Clinical Performance of Breast Cancer Tumor Markers on Novel Multiplex Immunoassay and Automatized LOCI Technology Platforms
Diagn.,
13
(19)
Astrocytes evoke a robust IRF7-independent type I interferon response upon neurotropic viral infection
J.Neuroinflamm.,
20
(1)
Social participation during the COVID-19 pandemic in persons with a high risk for a severe course of COVID-19 - results of a longitudinal, multi-center observational study in Germany
Health Psychol.Behav.Med.,
11
(1)
Mucosal-associated invariant T (MAIT) cells from Clostridioides difficile-infected patients exhibit a distinct proinflammatory phenotype and enhanced cytotoxic activity
Int.Immunol.
Amino acids, post-translational modifications, nitric oxide, and oxidative stress in serum and urine of long COVID and ex COVID human subjects
Amino.Acids
Proteomics reveals a global phenotypic shift of NK cells in HCV patients treated with direct-acting antivirals
Eur.J.Immunol.
Cell-Free Methylated PTGER4 and SHOX2 Plasma DNA as a Biomarker for Therapy Monitoring and Prognosis in Advanced Stage NSCLC Patients
Diagnostics.(Basel),
13
(13)
Applied biostatistics in laboratory medicine
J.Lab.Med.
The Cytomegalovirus M35 Protein Directly Binds to the Interferon-b[beta] Enhancer and Modulates Transcription of Ifnb1 and Other IRF3-Driven Genes
J.Virol.,
97
(6)
Correction to: Suggestion of a new standard in measuring the mandible via MRI and an overview of reference values in young women (Oral and Maxillofacial Surgery, (2023), 10.1007/s10006-023-01153-7)
Oral Maxillofac.Surg.
Mechanistic insights into the ROS-mediated inactivation of human aldehyde oxidase
FEBS Lett.
Feasibility of self-organized blood sample collection in adults for study purposes in a primary care setting
PLoS.ONE.,
18
(5)
Inactivation of Human Aldehyde Oxidase by Small Sulfhydryl-containing Reducing Agents
Drug Metab Dispos.
Corrigendum to Focusing on severe infections with the Respiratory Syncytial Virus (RSV) in adults: risk factors, symptomatology and clinical course compared to influenza A / B and the original SARS-CoV-2 strain (Journal of Clinical Virology (2023) 161, (S1386653223000227), (10.1016/j.jcv.2023.105399))
Journal of Clinical Virology
Cluster-Analytic Identification of Clinically Meaningful Subtypes in MCAS: The Relevance of Heat and Cold
Digestive Diseases and Sciences
Suggestion of a new standard in measuring the mandible via MRI and an overview of reference values in young women
Oral and Maxillofacial Surgery
Cell-Free DNA in Plasma and Serum Indicates Disease Severity and Prognosis in Blunt Trauma Patients
Diagnostics.(Basel),
13
(6)
A diagnostic support system based on pain drawings: binary and k-disease classification of EDS, GBS, FSHD, PROMM, and a control group with Pain2D
Orphanet.J.Rare.Dis.,
18
(1)
Longitudinal analysis of systemic ventricular function and atrioventricular valve function after the Fontan procedure
Eur.J.Cardiothorac.Surg.
Translatome analyses by bio-orthogonal non-canonical amino acid labeling reveal that MR1-activated MAIT cells induce an M1 phenotype and antiviral programming in antigen-presenting monocytes
Front.Immunol.,
14
HiPerMAb: A tool for judging the potential of small sample size biomarker pilot studies
Int.J.Biostat.
Focusing on severe infections with the respiratory syncytial virus (RSV) in adults: Risk factors, symptomatology and clinical course compared to influenza A / B and the original SARS-CoV-2 strain
J.Clin.Virol.,
161
GMP development and preclinical validation of CAR-T cells targeting a lytic EBV antigen for therapy of EBV-associated malignancies
Front.Immunol.,
14
Social participation and mental health of immunocompromised individuals before and after COVID-19 vaccination
Front.Psychiatry,
13
Moonlighting chaperone activity of the enzyme PqsE contributes to RhlR-controlled virulence of Pseudomonas aeruginosa
Nat.Commun.,
13
(1)
A zlog-based algorithm and tool for plausibility checks of reference intervals
Clin.Chem.Lab.Med.
Monitoring regional astrocyte diversity by cell type-specific proteomic labeling in vivo
Glia
Indian sewage microbiome has unique community characteristics and potential for population-level disease predictions
Sci.Total Environ.,
858
The autophagy inducer SMER28 attenuates microtubule dynamics mediating neuroprotection
Sci.Rep.,
12
(1)
Improving discretization based pattern discovery for multivariate time series by additional preprocessing
Intelligent Data Analysis,
25
(5)
Audiological profile of adult Long COVID patients
Am.J.Otolaryngol.Head Neck Med.Surg.,
43
(5)
Serum biomarker panel diagnostics in pancreatic ductal adenocarcinoma: the clinical utility of soluble interleukins, IFN-g[gamma], TNF-a[alpha] and PD-1/PD-L1 in comparison to established serum tumor markers
J.Cancer Res.Clin.Oncol.
The Impact of COVID-19 Vaccination on the Social Participation of Immunocompromised Persons - Results of a Multicenter Observational Study
Front Public Health,
10
Who is pausing immunosuppressive medication for COVID-19 vaccination? Results of an exploratory observational trial
Eur.J.Med.Res.,
27
(1)
Self-reported Tinnitus and Vertigo or Dizziness in a Cohort of Adult Long COVID Patients
Front.Neurol.,
13
Insights into polyamine metabolism: homospermidine is double-oxidized in two discrete steps by a single copper-containing amine oxidase in pyrrolizidine alkaloid biosynthesis
Plant Cell
An Antibody-Aptamer-Hybrid Lateral Flow Assay for Detection of CXCL9 in Antibody-Mediated Rejection after Kidney Transplantation
Diagn.,
12
(2)
A strict mask policy for hospital staff effectively prevents nosocomial influenza infections and mortality: monocentric data from five consecutive influenza seasons
J.Hosp.Infect.,
121
Clostridioides difficile Toxin CDT Induces Cytotoxic Responses in Human Mucosal-Associated Invariant T (MAIT) Cells
Front Microbiol.,
12
Rapid SARS-CoV-2 Adaptation to Available Cellular Proteases
J.Virol.
SARS-CoV-2 Antibody Testing - How to Increase the Positive Predictive Value
Clin.Lab.,
67
(12)
Editorial: Making an impact
Integr.Comput.Aided Eng.,
29
(1)
Influence of shunt type on survival and right heart function after the Norwood procedure for aortic atresia
Semin.Thorac.Cardiovasc.Surg.
Predicting Major Adverse Cardiovascular Events in Children With Age-Adjusted NT-proBNP
Journal of the American College of Cardiology,
78
(19)
In vivo lentiviral gene delivery of hla-dr and vaccination of humanized mice for improving the human t and b cell immune reconstitution
Biomedicines,
9
(8)
Human-relevant sensitivity of ipsc-derived human motor neurons to bont/a1 and b1
Toxins,
13
(8)
Development of a data generator for multivariate numerical data with arbitrary correlations and distributions
Intell.Data Anal.,
25
(4)
Haploflow: strain-resolved de novo assembly of viral genomes
Genome Biol.,
22
(1)
Pattern discovery in time series using autoencoder in comparison to nonlearning approaches
Integrated Computer-Aided Engineering,
28
(3)
Elevated Free Phosphatidylcholine Levels in Cerebrospinal Fluid Distinguish Bacterial from Viral CNS Infections
Cells,
10
(5)
Towards the characterization of the hidden world of small proteins in Staphylococcus aureus, a proteogenomics approach
PLoS.Genet.,
17
(6)
Proteogenomic characterization of the cement and adhesive gland of the pelagic gooseneck barnacle lepas anatifera
Int.J.Mol.Sci.,
22
(7)
Chemical conjugation of a purified dec-205-directed antibody with full-length protein for targeting mouse dendritic cells in vitro and in vivo
J.Visualized Exp.,
2021
(168)
Risk to Radiology Staff for Occupational COVID-19 Infection in a High-Risk and a Low-Risk Region in Germany: Lessons from the "First Wave"
Rofo,
193
(5)
A Point Mutation in the Transcriptional Repressor PerR Results in a Constitutive Oxidative Stress Response in Clostridioides difficile 630 D
mSphere.,
6
(2)
Guide to Intelligent Data Science : How to Intelligently Make Use of Real Data
,
2
(1-10)
Data analysis strategies for microbiome studies in human populations-a systematic review of current practice
mSystems.,
6
(1)
Quantitative laboratory results: Normal or lognormal distribution?
J.Lab.Med.,
44
(3)
The role of Saccharibacteria (TM7) in the subginival microbiome as a predictor for secondary cardiovascular events
Int.J.Cardiol.,
331
PD-1 Blockade Aggravates Epstein-Barr Virus(+) Post-Transplant Lymphoproliferative Disorder in Humanized Mice Resulting in Central Nervous System Involvement and CD4(+) T Cell Dysregulations
Front Oncol.,
10
Crystal structure of bacterial cytotoxic necrotizing factor CNF g
EMBO J,
40
(4)
Replicability, Repeatability, and Long-term Reproducibility of Cerebellar Morphometry
Cerebellum.
Dinoroseobacter shibae outer membrane vesicles are enriched for the chromosome dimer resolution site dif
mSystems.,
6
(1)
Hepatitis C reference viruses highlight potent antibody responses and diverse viral functional interactions with neutralising antibodies
GUT
SARS-CoV-2 antibodies in ICU and clinic staff: From Germany's region with the highest infection rate
Med.Klin.Intensivmed.Notf.med.,
115
(December)
Pain drawings as a diagnostic tool for the differentiation between two pain-associated rare diseases (Ehlers-Danlos-Syndrome, Guillain-Barr q
Orphanet J Rare Dis,
15
(1)
Mg-protoporphyrin IX monomethyl ester cyclase from Rhodobacter capsulatus: Radical SAM-dependent synthesis of the isocyclic ring of bacteriochlorophylls
Biochem.J.,
477
(23)
Effect of a strict hygiene bundle for the prevention of nosocomial transmission of SARS-CoV-2 in the hospital: a practical approach from the field
J.Infect.Public Health,
13
(12)
Sample size and performance estimation for biomarker combinations based on pilot studies with small sample sizes
Commun Stat Theory Methods
Development of a Social Network for People Without a Diagnosis (RarePairs): Evaluation Study
J Med.Internet.Res.,
22
(9)
Pyruvate dehydrogenase complex-enzyme 2, a new target for Listeria spp. detection identified using combined phage display technologies
Sci.Rep.,
10
(1)
CAR-T Cells Targeting Epstein-Barr Virus gp350 Validated in a Humanized Mouse Model of EBV Infection and Lymphoproliferative Disease
Mol.Ther.Oncolytics,
18
Clarithromycin Exerts an Antibiofilm Effect against Salmonella typhimurium rdar Biofilm Formation, and Transforms the Physiology towards an Apparent Oxygen-depleted Energy and Carbon Metabolism
Infect.Immun.,
88
(11)
Results of the first pilot external quality assessment (EQA) scheme for anti-SARS-CoV2-antibody testing
Clin.Chem.Lab Med.,
58
(12)
A publicly accessible database for Clostridioides difficile genome sequences supports tracing of transmission chains and epidemics
Microb.Genom.,
6
(8)
Repertoire characterization and validation of gB-specific human IgGs directly cloned from humanized mice vaccinated with dendritic cells and protected against HCMV
PLoS Pathog.,
16
(7)
Responsiveness to influenza vaccination correlates with nkg2c-expression on nk cells
Vaccines.(Basel),
8
(2)
Continuous, complete and comparable NT-proBNP reference ranges in healthy children
Clin.Chem.Lab Med.,
58
(9)
Crystal structure of NirF: Insights into its role in heme d1 biosynthesis
FEBS J.,
188
(1)
The quantitative proteome of the cement and adhesive gland of the pedunculate barnacle, pollicipes pollicipes
Int.J.Mol.Sci.,
21
(7)
ADAP Promotes Degranulation and Migration of NK Cells Primed During in vivo Listeria monocytogenes Infection in Mice
Front.Immunol.,
10
Phosphatidylcholine PC ae C44:6 in cerebrospinal fluid is a sensitive biomarker for bacterial meningitis
J.Transl.Med.,
18
(1)
Immune Monitoring of Human Mucosal-Associated Invariant T Cells by Quantitative Proteomics
Methods Mol.Biol.,
2098
(14)
Common pre-diagnostic features in individuals with different rare diseases represent a key for diagnostic support with computerized pattern recognition?
PLoS One.,
14
(10)
Two FtsH proteases contribute to fitness and adaptation of pseudomonas aeruginosa Clone C strains
Front.Microbiol.,
10
(JULY)
Immunoproteomic characterization of outer membrane vesicles from hyper-vesiculating Actinobacillus pleuropneumoniae
Vet.Microbiol.,
235
(Aug)
The nuclear export inhibitor aminoratjadone is a potent effector in extracellular-targeted drug conjugates
Chem.Sci.,
10
(20)
Indirekte Überprüfung von Referenzintervallen - Leberwerte in neuem Licht
Trillium Diagnostik,
2018
(2)
Pleiotropic Clostridioides difficile Cyclophilin PpiB Controls Cysteine-Tolerance, Toxin Production, the Central Metabolism and Multiple Stress Responses
Front Pharmacol.,
10
(4)
Artificial intelligence for diagnostic support in selected rare lysosomal storage disorders: Results of a pilot study
Klin.Padiatr.,
231
(2)
N-Glycosylation of Extracellular Vesicles from HEK-293 and Glioma Cell Lines
Anal.Chem.,
90
(13)
Letter to the editor by Winter et al.: Reply
J.Lab.Med.,
42
(1-2)
DncV Synthesizes Cyclic GMP-AMP and Regulates Biofilm Formation and Motility in Escherichia coli ECOR31
MBio,
10
(2)
Modulation of TAP-dependent antigen compartmentalization during human monocyte-to-DC differentiation
Blood Adv.,
3
(6)
xCELLanalyzer: A Framework for the Analysis of Cellular Impedance Measurements for Mode of Action Discovery
SLAS.Discov.
Exploring Cerebrospinal Fluid IgG N-Glycosylation as Potential Biomarker for Amyotrophic Lateral Sclerosis
Mol Neurobiol.,
56
(8)
Bacterial community structure and effects of picornavirus infection on the anterior nares microbiome in early childhood
BMC.Microbiol.,
19
(1)
Künstliche Intelligenz zur diagnostischen Unterstützung ausgewählter seltener lysosomaler Speichererkrankungen: Ergebnisse einer Pilotstudie. [Artificial intelligence for diagnostic support in selected rare lysosomal storage disorders: Results of a pilot study]
Klin.Padiatr.,
231
(2)
Improving the Decision Support in Diagnostic Systems Using Classifier Probability Calibration
LNCS (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)),
11314 LNCS
(44)
Bacterial load and pathogenic species on healthcare personnel attire: implications of alcohol hand-rub use, profession, and time of duty
J Hosp Infect,
101
(4)
Clostridioides difficile activates human mucosal-associated invariant t cells
Front.Microbiol.,
9
(OCT)
Hyfraxins A and B, cytotoxic ergostane-type steroid and lanostane triterpenoid glycosides from the invasive ash dieback ascomycete Hymenoscyphus fraxineus
Steroids,
135
(July)
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition
J.Neuroinflamm.,
15
(1)
Fate of the UPR marker protein Kar2/Bip and autophagic processes in fed-batch cultures of secretory insulin precursor producing Pichia pastoris
Microb.Cell Fact.,
17
(1)
Proteome analysis of human CD56(neg) NK cells reveals a homogeneous phenotype surprisingly similar to CD56(dim) NK cells
Eur.J.Immunol.,
48
(9)
External quality assessment schemes for glucose measurements in Germany: Factors for successful participation, analytical performance and medical impact
Clin.Chem.Lab.Med.,
56
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Neurobeachin and the Kinesin KIF21B Are Critical for Endocytic Recycling of NMDA Receptors and Regulate Social Behavior
Cell Rep.,
23
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A phosphatidic acid binding protein is important for lipid homeostasis and adaptation to anaerobic biofilm conditions in Pseudomonas aeruginosa
Biochem.J.,
475
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Proteomic definition of human mucosal-associated invariant T cells determines their unique molecular effector phenotype
Eur.J.Immunol.,
48
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Analysis and design of stimulus response curves of E. coli
Metabolites,
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Properties of dimeric, disulfide-linked rhBMP-2 recovered from E. coli derived inclusion bodies by mild extraction or chaotropic solubilization and subsequent refolding
Process Biochem.,
67
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The interferon-stimulated gene product oligoadenylate synthetase-like protein enhances replication of Kaposi's sarcoma-associated herpesvirus (KSHV) and interacts with the KSHV ORF20 protein
PLoS.Pathog.,
14
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[Clinical Characteristics and Course of Infections by Influenza A- and Respiratory Syncytial Virus (RSV) in Hospitalized Adults] Symptomatologie und klinischer Verlauf bei hospitalisierten Erwachsenen mit Virusinfektionen durch Influenza A und Respiratory Syncytial-Virus (RSV)
Dtsch.Med.Wochenschr.
Modulating the Molybdenum Coordination Sphere of Escherichia coli Trimethylamine N-Oxide Reductase
Biochemistry,
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Mass-spectrometric profiling of cerebrospinal fluid reveals metabolite biomarkers for CNS involvement in varicella zoster virus reactivation
J.Neuroinflammation.,
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HAUCA curves for the evaluation of biomarker pilot studies with small sample sizes and large numbers of features
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics,
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Effects of pathogen dependency in a multi-pathogen infectious disease system including population level heterogeneity - a simulation study
Theor.Biol.Med.Model.,
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Evaluation of statistical parameters of concrete strength from secondary experimental test data
Constr Build Mater,
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Stand-alone ClpG disaggregase confers superior heat tolerance to bacteria
Proc.Natl.Acad.Sci.U.S.A,
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Exploring time-resolved data for patterns and validating single clusters
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Eur.J.Cell Biol.,
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TCR signalling network organization at the immunological synapses of murine regulatory T cells
Eur.J.Immunol.,
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An international investigation into O red blood cell unit administration in hospitals: the GRoup O Utilization Patterns (GROUP) study
Transfusion,
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Global proteome response of Escherichia coli BL21 to production of human basic fribroblast growth factor in complex and defined medium
Engineering in Life Sciences,
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Diagnosis support for orphan diseases - a case study using a classifier fusion method
The Guanine-Nucleotide Exchange Factor Caldag Gefi Fine-Tunes Functional Properties of Regulatory T Cells
Eur.J.Microbiol.Immunol.(Bp),
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Chronic lung inflammation primes humoral immunity and augments antipneumococcal resistance
Sci.Rep.,
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Stronger Together: Aggregated Z-values of Traditional Quality Control Measurements and Patient Medians Improve Detection of Biases
Clin.Chem.,
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CTLA-4-mediated posttranslational modifications direct cytotoxic T-lymphocyte differentiation
Cell Death.Differ.,
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Iron affinity gel and gallium immobilized metal affinity chromatographic technique for phosphopeptide enrichment: a comparative study
Biotechnol.Biotechnol.Equip.,
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Hematological parameters in the early phase of influenza A virus infection in differentially susceptible inbred mouse strains Infectious Diseases
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Relative ion intensities of maltooligosaccharide ethers in electrospray ionization ion trap mass spectrometry: A quantitative evaluation
Int.J.Mass Spectrom.,
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Bundesgesundheitsblatt.Gesundheitsforschung.Gesundheitsschutz.,
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Characterization and genome comparisons of three Achromobacter phages of the family Siphoviridae
Arch.Virol.,
162
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Diagnostic needs for rare diseases and shared prediagnostic phenomena: Results of a German-wide expert Delphi survey
PLoS ONE,
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[The zlog-value as basis for the standardization of laboratory results] Der zlog-Wert als Basis für die Standardisierung von Laborwerten
LaboratoriumsMedizin,
41
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Effects of drift and noise on the optimal sliding window size for data stream regression models
Commun Stat Theory Methods,
46
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Alignment of time-resolved data from high throughput experiments
J.Bioinformatics Comput.Biol.,
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Analysis of practical identifiability of a viral infection model
PLoS ONE,
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Exploring Data Sets for Clusters and Validating Single Clusters
Procedia Computer Science,
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Farnesyl biliverdins IXa are novel ligands of biliproteins from moths of the Noctuoidea superfamily: A chemosystematic view of the Lepidoptera
Insect Biochem.Mol.Biol.,
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Transcriptome-Wide High-Density Microarray Analysis Reveals Differential Gene Transcription in Periprosthetic Tissue From Hips With Chronic Periprosthetic Joint Infection vs Aseptic Loosening
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Listeriosis down-regulates hepatic cytochrome P450 enzymes in sub-lethal murine infection
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Transcriptome profiling of antimicrobial resistance in Pseudomonas aeruginosa
Antimicrobial Agents Chemother.,
60
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The Molybdenum Active Site of Formate Dehydrogenase Is Capable of Catalyzing C-H Bond Cleavage and Oxygen Atom Transfer Reactions
Biochemistry,
55
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What can fuzzy cluster analysis contribute to clustering of high-dimensional data?
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics),
8256 LNAI
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IT support in the diagnosis of rare diseases - Shorter diagnostic pathways and rapid data clustering in complicated cases [IT-Unterstützung zur Diagnosefindung seltener Erkrankungen - Umfassende Datenanalyse bei komplexen Fällen]
Der Klinikarzt,
44
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What are clusters in high dimensions and are they difficult to find?
Lecture Notes in Computer Science
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VoQs: A web application for visualization of questionnaire surveys
Lecture Notes in Computer Science,
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LC/MS based monitoring of endogenous decay markers for quality assessment of serum specimens
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Diagnostic support for selected neuromuscular diseases using answer-pattern recognition and data mining techniques: a proof of concept multicenter prospective trial
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The Protein Network of the Pseudomonas aeruginosa Denitrification Apparatus
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Mixture Effects of Estrogenic Pesticides at the Human Estrogen Receptor a[alpha] and b[beta]
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The ability of the YAS and AR CALUX assays to detect the additive effects of anti-androgenic fungicide mixtures
Toxicol.Lett.,
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Very high-density lipoprotein and vitellin as carriers of novel biliverdins IXa[alpha] with a farnesyl side-chain presumably derived from heme A in Spodoptera littoralis
Insect Biochem.Mol.Biol.,
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Fuzzy clustering: More than just fuzzification
Fuzzy Sets Syst,
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The LasB elastase of Pseudomonas aeruginosa acts in concert with alkaline protease AprA to prevent flagellin-mediated immune recognition
Infect.Immun.,
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Application of synthetic peptide arrays to uncover cyclic Di-GMP binding motifs
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198
A periplasmic complex of the nitrite reductase NirS, the chaperone DnaK, and the flagellum protein FliC is essential for flagellum assembly and motility in Pseudomonas aeruginosa
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Linear Discriminant Analysis Identifies Mitochondrially Localized Proteins in Neurospora crassa
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Optimized leaf ordering with class labels for hierarchical clustering
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Incremental statistical measures
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Prototype based fuzzy clustering algorithms in high-dimendional features spaces
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The aerobic respiratory chain of the acidophilic archaeon Ferroplasma acidiphilum: A membrane-bound complex oxidizing ferrous iron
Biochim.Biophys.Acta,
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Enzymatic characterization of recombinant nitrate reductase expressed and purified from Neurospora crassa
Fungal Genet.Biol.,
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Lipopolysaccharide binding protein, interleukin-10, interleukin-6 and C-reactive protein blood levels in acute ischemic stroke patients with post-stroke infection
J.Neuroinflamm.,
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Manganese Peroxidases from Ganoderma applanatum Degrade b[beta]-Carotene Under Alkaline Conditions
Appl.Biochem.Biotechnol.,
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Primary Osteomyelitis of the Sternum in the Pediatric Age Group: Report of a New Case and Comprehensive Analysis of Seventy-four Cases
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Differential control of immune cell homeostasis by Foxp3(+) regulatory T cells in murine peripheral lymph nodes and spleen
Eur.J Microbiol.Immunol.(Bp),
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Single-cell analysis reveals heterogeneity in onset of transgene expression from synthetic tetracycline-dependent promoters
Biotechnol.J.,
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Carotene-degrading activities from Bjerkandera adusta possess an application in detergent industries
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The lack of type I interferon induces neutrophil-mediated pre-metastatic niche formation in the mouse lung
International Journal of Cancer,
137
High-resolution taxonomic profiling of the subgingival microbiome for biomarker discovery and periodontitis diagnosis
Appl.Environ.Microbiol.,
81
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The human otubain2-ubiquitin structure provides insights into the cleavage specificity of poly-ubiquitin-linkages
PLoS ONE,
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Relative cost curves: An alternative to AUC and an extension to 3-class problems
Kybernetika,
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HOPE-fixation of lung tissue allows retrospective proteome and phosphoproteome studies
Journal of Proteome Research,
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The Treg-specific demethylated region stabilizes Foxp3 expression independently of NF-?B signaling
PLoS ONE,
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Balticidins A-D, antifungal hassallidin-like lipopeptides from the Baltic Sea cyanobacterium Anabaena cylindrica Bio33
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An alcohol oxidase of Phanerochaete chrysosporium with a distinct glycerol oxidase activity
Enzyme Microbial Technology,
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Structure of a novel farnesylated bilin from an insect - Formation by ?-cleavage of heme A of mitochondrial cytochrome c oxidases?
FEBS Journal,
281
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Membrane Proteomics of Pseudomonas aeruginosa
Methods in Molecular Biology,
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Cross-feeding and interkingdom communication in dual-species biofilms of Streptococcus mutans and Candida albicans
ISME Journal,
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Baceridin, a cyclic hexapeptide from an epiphytic bacillus strain, inhibits the proteasome
ChemBioChem,
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HUM calculator and HUM package for R: easy-to-use software tools for multicategory receiver operating characteristic analysis
Bioinformatics,
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A halotolerant type A feruloyl esterase from Pleurotus eryngii
Fungal Biology,
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Field exposure and dosimetry in the thz frequency range
IEEE Transactions on Terahertz Science and Technology,
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Infection- and procedure-dependent effects on pulmonary gene expression in the early phase of influenza A virus infection in mice
BMC Microbiology,
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Genetic characterization of the Neurospora crassa molybdenum cofactor biosynthesis
Fungal Genetics and Biology,
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Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures
Microbial Cell Factories,
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Decision and regression trees in the context of attributes with different granularity levels
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Handling different levels of granularity within naive Bayes classifiers
Lecture Notes in Computer Science,
8206 LNCS
High against low quantile comparison for biomarker and classifier evalution
Lecture Notes in Computer Science,
8206 LNCS
Human lung tissue explants reveal novel interactions during Legionella pneumophila infections
Infection and Immunity,
82
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An enzyme from Auricularia auricula-judae combining both benzoyl and cinnamoyl esterase activity
Process Biochemistry,
48
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Amyloid arthropathy associated with multiple myeloma: A systematic analysis of 101 reported cases
Seminars in Arthritis and Rheumatism,
43
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Time point estimation of a single sample from high throughput experiments based on time-resolved data and robust correlation measures
LNCS (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)),
8207
Peptidases released by necrotic cells control CD8+ T cell cross-priming
Journal of Clinical Investigation,
123
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Analysis of contingency tables based on generalized median polish with power transformations and non-additive models
Health Information Science & Systems,
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Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes
Microbial Cell Factories,
11
Involvement of the mitogen activated protein kinase Hog1p in the response of Candida albicans to iron availability
BMC Microbiology,
13
The relative composition of the inflammatory infiltrate as an additional tool for synovial tissue classification
PLoS ONE,
8
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Regulatory and Metabolic Networks for the Adaptation of Pseudomonas aeruginosa
PLoS ONE,
8
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An esterase from the basidiomycete Pleurotus sapidus
Applied Microbiology and Biotechnology,
97
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Testing noisy numerical data for monotonic association
Information Sciences,
245
Maturation of the cytochrome cd1 nitrite reductase NirS from Pseudomonas aeruginosa requires transient interactions between the three proteins NirS, NirN and NirF
Bioscience Reports,
33
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Case-centred multidimensional scaling for classification visualisation in medical diagnosis
LNCS,
7798
The sulfur carrier protein TusA has a pleiotropic role in Escherichia coli
Journal of Biological Chemistry,
288
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Proteome analysis of distinct developmental stages of human natural killer (NK) cells
Molecular and Cellular Proteomics,
12
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PfaH2: A novel hydrophobin from the ascomycete Paecilomyces farinosus
Biotechnology and Applied Biochemistry,
60
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A direct in vivo RNAi screen identifies MKK4 as a key regulator of liver regeneration
Cell,
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Distinct gene loci control the host response to influenza H1N1 virus infection in a time-dependent manner
BMC GENOMICS,
13
A New Distance Function for Fuzzy c-Means Clustering in High-Dimensional Spaces with Applications in S.O.D.A.
Change Detection Based on the Distribution of p-Values
Protein Kinase Inhibitors CK59 and CID755673 Alter Primary Human NK Cell Effector Functions
Frontiers in Immunology,
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Terahertz Radiation at 0.380 THz and 2.520 THz Does Not Lead to DNA Damage in Skin Cells In Vitro
Radiation Research,
179
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The peptide chain release factor methyltransferase PrmC is essential for pathogenicity and environmental adaptation of Pseudomonas aeruginosa PA14
Environmental Microbiology,
15
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Should paediatricians use a computer to receive diagnostic support? [Sollten Kinderärzte zur Unterstützung bei der Diagnostik einen Computer nutzen?]
Pediatrische Praxis,
79
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Characterization of an antifungal compound produced by Bacillus sp. strain A(5) F that inhibits Sclerotinia sclerotiorum
Journal of Basic Microbiology,
52
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Can unbounded distance measures mitigate the curse of dimensionality?
International Journal of Data Mining, Modelling and Management,
4
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Detecting glycosylations in complex samples
IFIP Advances in Information and Communication Technology,
381 AICT (Part 1)
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Generalised median Polish based on additive generators
Advances in Intelligent Systems and Computing,
190 AISC
Curve fitting for short time series data from high throughput experiments with correction for biological variation
Lecture Notes in Computer Science,
LNCS 7619
Can fuzzy clustering avoid local minima and undesired partitions?
Studies in Computational Intelligence,
445
Terahertz Electromagnetic Fields (0.106 THz) Do Not Induce Manifest Genomic Damage In Vitro
PLoS ONE,
7
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Significance tests to identify regulated proteins based on a large number of small samples
Kybernetika,
48
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Kinome analysis of receptor-induced phosphorylation in human natural killer cells
PLoS ONE,
7
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Comparative genomics and transcriptomics of lineages I, II, and III strains of Listeria monocytogenes
BMC GENOMICS,
13
Urine Proteome Analysis Reflects Atherosclerotic Disease in an ApoE-/- Mouse Model and Allows the Discovery of New Candidate Biomarkers in Mouse and Human Atherosclerosis
Molecular and Cellular Proteomics,
11
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First insight into the kinome of human regulatory T cells
PLoS ONE,
7
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Profiling ubiquitin linkage specificities of deubiquitinating enzymes with branched ubiquitin isopeptide probes
ChemBioChem,
13
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Tackling misleading peptide regulation fold changes in quantitative proteomics
Advances in Intelligent and Soft Computing,
154
In vitro field exposition of skin cells between 100 GHz and 2.52 THz
Identification and localization of a lipase-like acyltransferase in phenylpropanoid metabolism of tomato (Solanum lycopersicum)1
Journal of Biological Chemistry,
285
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A novel oxygenase from Pleurotus sapidus transforms valencene to nootkatone
Journal of Molecular Catalysis B: Enzymatic,
61
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Median polish with power transformations as an alternative for the analysis of contingency tables with patient data
LNCS (Lecture Notes in Computer Sience (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)),
7231
Resistance phenotypes mediated by aminoacyl-phosphatidylglycerol synthases
Journal of Bacteriology,
194
(6)
SignalNet: Visualization of signal network responses by quantitative proteome data
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics,
6936 LNCS
Evaluation of glyceraldehyde-3-phosphate, prolylpeptidyl isomerase A, and a set of stably expressed genes as reference mRNAs in urate crystal inflammation
BMC Research Notes,
4
Complete genome sequence of the animal pathogen Listeria ivanovii, which provides insights into host specificities and evolution of the genus Listeria1
Journal of Bacteriology,
193
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Identification of persulfide-binding and disulfide-forming cysteine residues in the NifS-like domain of the molybdenum cofactor sulfurase ABA3 by cysteine-scanning mutagenesis
Biochemistry Journal,
441
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Nitrate-responsive NarX-NarL represses arginine-mediated induction of the Pseudomonas aeruginosa arginine fermentation arcDABC operon1
Microbiology,
154
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A chemical proteomics approach to identify c-di-GMP binding proteins in Pseudomonas aeruginosa
Journal of Microbiological Methods,
88
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An orphan sensor kinase controls quinolone signal production via MexT in Pseudomonas aeruginosa
Molecular Microbiology,
83
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A modified apriori algorithm for analysing high-dimensional gene data
LNCS,
6936
Elucidation of the Dual Role of Mycobacterial MoeZR in Molybdenum Cofactor Biosynthesis and Cysteine Biosynthesis
PLoS ONE,
6
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Identification of nuclear magnetic resonance signals via Gaussian mixture decomposition
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics),
7014 LNCS
An alternative to ROC and AUC analysis of classifiers
LNCS (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)),
7014
The luxS mutation causes loosely-bound biofilms in Shewanella oneidensis
BMC Research Notes,
4
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The extracellular aspartic peptidase of basidiomycete Wolfiporia cocos
Milchwissenschaft,
66
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The Identification of a Novel Protein Involved in Molybdenum Cofactor Biosynthesis in Escherichia coli
Journal of Biological Chemistry,
286
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Identification of peptides with deviating regulation factors using a robust clustering scheme
Advances in Intelligent and Soft Computing,
93
PsoP1, a Milk-Clotting Aspartic Peptidase from the Basidiomycete Fungus Piptoporus soloniensis
Journal of Agricultural and Food Chemistry,
59
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Cluster validity measures based on the minimum description length principle
Lecture Notes in Computer Science,
6881 LNAI (part 1)
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Heterologous expression, refolding and characterization of a salt activated subtilase from Pleurotus ostreatus
Process Biochemistry,
46
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Characterization of a hydrophobin of the ascomycete Paecilomyces farinosus
Journal of Basic Microbiology,
51
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High-resolution mass spectrometric analysis of the secretome from mouse lung endothelial progenitor cells
Angiogenesis,
14
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Biosynthesis of the repeating units of the exopolysaccarides amylovoran from Erwinia amylovora
Physiological and Molecular Plant Pathology,
75
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Alanyl-phosphatidylglycerol synthase: mechanism of substrate recognition during tRNA-dependent lipid modification in Pseudomonas aeruginosa
Molecular Microbiology,
80
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Diagnosis of subclinical and clinical acute T-cell-mediated rejection in renal transplant patients by urinary proteome analysis
Proteomics -Clinical Applications,
5
(5-6)
Plastid gene expression and plant development require a plastidic protein of the mitochondrial transcription termination factor family
Proceedings of the National Academy of Sciences USA,
108
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GeneReporter--sequence-based document retrieval and annotation
Bioinformatics,
27
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Saliva proteins of vector culicoides modify structure and infectivity of bluetongue virus particles
PLoS ONE,
6
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Mining quorum sensing regulated proteins - role of bacterial cell-to-cell communication in global gene regulation as assessed by proteomics
Proteomics,
11
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Fuzzy clustering with polynomial fuzzifier function in connection with m-estimators
Applied and Computational Mathematics,
10
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The intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate
Microbial Cell Factories,
9
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The first characterized asparaginase from a basidiomycete, Flammulina velutipes
Bioresource Technology,
102
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The need for benchmarks with data from stochastic processes and meta-models in evolving systems
Rank correlation coefficient correction by removing worst cases
Evaluation of Madurahydroxylactone as a Slow Release Antibacterial Implant Coating
Open Biomedical Engineering Journal,
4
Anaerobic adaptation in Pseudomonas aeruginosa
Environmental Microbiology,
12
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Incremental quantile estimation
Evolving Systems,
1
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Over-expression of PR-10a leads to increased salt and osmotic tolerance in potato cell cultures
Journal of Biotechnology,
150
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History-dependent catastrophes regulate axonal microtubule behavior
Current biology,
20
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Urinary collagen fragments are significantly altered in diabetes: a link to pathophysiology
PLoS ONE,
5
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Global genotype-phenotype correlations in Pseudomonas aeruginosa
PLoS Pathogens,
6
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Columnar liquid crystals derived from crown ethers with two lateral ester-substituted ortho-terphenyl units: Unexpected destabilisation of the mesophase by potassium iodide
Liquid Crystals,
37
(9)
Comprehensive human urine standards for comparability and standardization in clinical proteome analysis
Proteomics - Clinical Applications,
4
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Internal architecture of mitochondrial complex I from Arabidopsis thaliana
Plant Cell,
22
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Quantitative phosphokinome analysis of the met pathway activated by the invasin internalin B from Listeria monocytogenes
Molecular and Cellular Proteomics,
8
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AVEDA: Statistical tests for finding interesting visulatisations
5711 LNAI (Part 1),
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics
Clustering likelihood curves: Finding deviations from single clusters
Compensation of translational displacement in time series clustering using cross correlation
Lecture Notes in Computer Science,
(57772 LCNS)
Genomewide identification of genetic determinants of antimicrobial drug resistance in Pseudomonas aeruginosa
Antimicrobial Agents and Chemotherapy,
53
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Heterologous expression of an extra-cellular lipase from the basidiomycete Pleurotus sapidus
Journal of Molecular Catalysis B: Enzymatic,
57
MS-specific noise model reveals the potential of iTRAQ in quantitative proteomics
Bioinformatics,
25
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IRSp53 links the enterohemorrhagic E. coli effectors Tir and EspFU for actin pedestal formation
Cell Host and Microbe,
5
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Quantitative gel electrophoresis: sources of variation
Journal of Proteome Research,
7
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ProdoNet: identification and visualization of prokaryotic gene regulatory and metabolic networks
Nucleic Acids Research,
36
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Lipoproteins of Listeria monocytogenes are critical for virulence and TLR2-mediated immune activation
Journal of Immunology,
181
(3)
Structure of xylogalacturonan fragments from watermelon cell-wall pectin. Endopolygalacturonase can accommodate a xylosyl residue on the galacturonic acid just following the hydrolysis site
Carbohydrate Research,
343
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Mitochondrial cytochrome c oxidase and succinate dehydrogenase complexes contain plant specific subunits
Plant Molecular Biology,
56
(1)
Enzymes involved in anaerobic respiration appear to play a role in Actinobacillus pleuropneumoniae virulence
Infection and Immunity,
73
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Proteomic characterization of the angiogenesis inhibitor SU6668 reveals multiple impacts on cellular kinase signaling
Cancer Research,
65
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A structural investigation of complex I and I+III(2) supercomplex from Zea mays at 11-13 A resolution: Assignment of the carbonic anhydrase domain and evidence for structural heterogeneity within complex I
Biochimica et biophysica acta,
1777
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A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium
Journal of Biotechnology,
132
(4)
Elucidation of mechanisms of acid stress in Listeria monocytogenes
The cell wall subproteome of Listeria monocytogenes
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Proteomic analysis of the U1 snRNP of Schizosaccharomyces pombe
Nucleic Acids Research,
35
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Proteomics analysis of protein kinases by target class-selective pre-fractionation and tandem mass spectrometry
Molecular & Cellular Proteomics,
6
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Inactivation of Lgt allows systematic characterization of lipoproteins from Listeria monocytogenes
Journal of Bacteriology,
189
(2)
Quantitative analysis of receptor tyrosine kinase signaling exploited by Listeria monocytogenes
Molecular & Cellular Proteomics,
5
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Direct mass spectrometric peptide profiling and fragmentation of larval peptide hormone release sites in Drosophila melanogaster reveals tagma-specific peptide expression and differential processing
Journal of Neurochemistry,
96
(5)
Carbonic anhydrase subunits form a matrix-exposed domain attached to the membrane arm of mitochondrial complex I in plants
Journal of Biological Chemistry,
281
(10)
The Pseudomonas aeruginosa
Journal of Bacteriology,
188
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LEGER: knowledge database and visualization tool for comparative genomics of pathogenic and non-pathogenic Listeria species
Nucleic Acids Research,
34
Anaerobic survival of Pseudomonas aeruginosa
Journal of Bacteriology,
188
Characterization of the Drosophila Lipid Droplet Subproteome
Molecular & Cellular Proteomics,
5
The MprF protein is required for lysinylation of phospholipids in listerial membranes and confers resistance to cationic antimicrobial peptides (CAMPs) on Lysteria monocytogenes
Molecular Microbiology,
62
The substrate radical of Escherichia coli
Journal of Biological Chemistry,
281
Quorum-sensing antagonistic activities of azithromycin in Pseudomonas aeruginosa
Antimicrobial Agents and Chemotherapy,
50
VIS-O-BAC: exploratory visualization of functional genome studies from bacteria
Bioinformatics,
22
(5)
MineBlast: A literature presentation service supporting protein annotation by data Mining of blast results
Bioinformatics,
21
Comparative proteome analysis of secretory proteins from pathogenic and non-pathogenic Listeria species
Proteomics,
5
Systematic identification of covalently-bound cell wall proteins and analysis of protein-polysaccharide linkages of the human pathogen Candida glabrata
Microbiology,
150
Projekte
mit Schwerpunkt auf mikrovesikulären Transport (Projekt B16, SFB854, mit Prof. B. Schraven, Prof. A Müller, Dr. S. Kliche, Dr. W. Zuschratter, Universität Magdeburg
Charakterisierung von humanen Mukosa-assoziierten invarianten T Zellen in Clostridium difficile-Infektionen (ABINEP & Land Niedersachsen, mit Prof. D. Jahn, TU-BS, Prof. D. Bruder, Universität Magdeburg, Prof. A. Canbay, Klinikum Magdeburg)
Charakterisierung der humanen NK-Zell vermittelten Immunität (iMED)
- in HCV-Infektionen und Therapie
(mit Prof. H. Wedemeyer, Universität Essen; Prof. M. Cornberg, Mdizinische Hochschule Hannover; Prof. H.G. Ljunggren, Prof. J. Sandberg, Karolinska, SE; Prof. E. Mace, Baylor College of Medicine, US) - in HIV-infizierten Patienten
(mit Prof. J. Sandberg, Karolinska, SE und Dr. M. Eller, MHRP, US).
Atmungskettenkomplex (respirasome) Dynamik in aktivierten T-Lymphozyten mit Schwerpunkt auf ROS-abhängiger mukosaler Immunität (DFG Graduiertenschule Proteinkomplex Assemblierung - PROCOMPAS, mit Prof. R. Mendel)
Wirkstoff-Zielprotein-Identifizierungen und Aufklärung von Wirkmechanismen mittels Proteomik im Rahmen der Helmholtz Wirkstoffforschungsinitiative: Wirkstoffforschung
Exosom-Charakterisierung im Rahmen von Norovirus-Infektionen als Teil des Big-Data-Konsortiums „PRESENt“: Personalisierte Vorsorge und Pflege bei schwere Norovirus Gastroenteristis (neu)
Plasma-Proteomanalytik und Immunzell-Monitoring im Rahmen des Big Data Konsortiums „i.Vacc“: Entwicklung individualisierter Impfungen (neu)
Ehemalige Projekte
De-ubiquitin protease mediated immune control in SIRS and Sepsis (iMED; with Prof. D. Schlüter, University Magdeburg and Dr. U. Lodes ICU Magdeburg).
EBV-specific alterations of the ubiquitin system in the process of B cell transformation (with Prof. H. Delecluse, DKFZ, Heidelberg).
Bioinformatik und Statistik
Dieses Projekt bietet für das gesamte HZI Unterstützung und Zusammenarbeit in den Bereichen Biostatistik und Bioinformatik an.
Bei der Proteomdatenanalyse wird besonders viel Wert auf robuste statistische Methoden gelegt, die mit Unsicherheiten umgehen können, die durch biologische Variation und verrauschte Messungen entstehen können. Insbesondere liegen die Schwerpunkte auf den folgenden wissenschaftlichen Fragestellungen:
- Identifikation von Pathogen-/Spender-/ oder Untergruppen-spezifischen Proteinen
- Erkennung von Mustern als Biomarker
- Auswertung zeitaufgelöster Daten.
Ansprechpartner
Prof. Dr. Frank Klawonn
Projektleiter
Zelluläre Proteomforschung
HZI Campus
Proteomanalytik
Diese analytische Plattform unterstützt Proteomforschung im Rahmen kooperativer Projekte. Wir kombinieren hierfür Expertise in Biochemie, Massenspektrometrie (MS) und Biostatistik, und entsprechen damit den Anforderungen von translationaler Forschung und Forschungsansätzen auf Systemebene. Unsere Forschungs- und Entwicklungsarbeiten konzentrieren sich auf die Charakterisierung von Proteinen, die eine wichtige Funktion in Infektionen haben und bevorzugte Ziele in der Wirkstoffforschung sind. Die Plattform trägt zur Identifizierung neuer Zielproteine bei, die in Pathogen-Wirt-Interaktionen und immunologischen Reaktionen entscheidend den Verlauf von Infektionsprozessen koordinieren.
Wir unterstützen:
- De novo-Proteinanalytik (ETD/MSn)
- Quantitative Proteomanalysen (TMT, iTRAQ, SILAC)
- Molekulare Phänotypisierung von Geweben und Zellen
- Generierung von Spender- und Patienten-spezifischen Proteomprofilen
- Interaktom-Studien (IP-MS)
- Analyse post-translationaler Proteinmodifikationen (u.a. Phosphorylierung, Ubiquitinierung, Glykosylierung)
- Identifizierung von Proteinen für Wirkstoffentwicklungen
- In situ Proteomik mittels Laser-Mikrodissektionsmikroskopie (neu)
Equipment
Die Plattform nutzt moderne Verfahren der Massenspektromerie (MS) und Proteomik einschließlich Orbitrap MS (Velos Pro & ETD-Fusion), SILAC-, iTRAQ- und TMT-Technologien sowie Analysesoftware und Datenbanken (z.B. ProteomDiscoverer, MaxQuant und Peaks Studio).
Für Projekte im Bereich Klinische Proteomik und Kohortenstudien (Epidemiologie) steht zudem Ionenmobilitäts-Massenspektrometrie (timsTOF pro) inklusive automatischer Probenvorbereitung (BRAVO, Evosep) zur Verfügung. Die Analyse komplexer Datensätze („big data“) in klinischen und translationalen Projekten wird durch die Gruppe von Prof. Frank Klawonn unterstützt.
Darüber hinaus sind in situ Analysen von Infektionsprozessen mittels Laser-Dissektionsmikroskopie (Palm, Zeiss) in dieser MS Einheit möglich. Wir unterstützen die Analyse von subzellulären Strukturen oder Gewebebereichen für alle Omic-Technologien (Kooperation Prof. Theresia Stradal). Proteomanalysen können ab 10.000 sortierten Zellen durchgeführt werden.
Sind Sie Kliniker oder Forscher in den Bereichen Infektion, Immunologie bzw. Wirkstoffforschung mit Bedarf an Proteomanalysen?
ANSPRECHPARTNER
- Dr. Manfred Nimtz (de novo & PTM Proteinanalytik)
- Dr. Josef Wissing (Proteomanalysen & Interaktomik)
- Dr. Marco vanHam (in situ-Proteomanalysen)
- Prof. Dr. Lothar Jänsch
Synaptic Systems
Synaptic Systems GmbH (SySy) entwickelt und produziert Antikörper höchster Qualität. Die Firma mit Sitz in Göttingen ist sehr an kooperativen Forschungsprojekten interessiert. Die Einheit „Monoklonale Antikörper“ arbeitet unmittelbar auf dem HZI Campus und unterstützt verschiedene infektionsbiologische Projekte. Insbesondere werden Antikörper für die Mauspathologie und die Charakterisierung von Proteinen der immunologischen und neurologischen Synapsen entwickelt und zur Verfügung gestellt. Die monoklonalen Antikörper von SySy sind speziell geeignet für die Analyse von Geweben, die in Paraffin eingebettet sind, was eine molekulare und zelluläre Phenotypisierung in situ ermöglicht.
Kontakt
Dr. Christian Erck
Wissenschaftler
Zelluläre Proteomforschung
HZI Campus
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Bachelor- & Masterarbeiten
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