Prof. Dr. Dagmar Wirth

Sarker S., Köster M., Desai O., Rahim M.I., Herrmann S., Behme S., Stiesch M., ... , Hauser H., Wirth D. (2024)

A generic cell-based biosensor converts bacterial infection signals into chemoattractants for immune cells

Biofabrication., 17 (1)

DOI: 10.1088/1758-5090/ad8bf4 HZI-Repositorium PubMed

Keles M., Grein S., Froese N., Wirth D., Trogisch F.A., Wardman R., Hemanna S., Weinzierl N., Koch P.S., Uhlig S., Lomada S., Dittrich G.M., Szaroszyk M., Haustein R., Hegermann J., Martin-Garrido A., Bauersachs J., Frank D., Frey N., Bieback K., Cordero J., Dobreva G., ... , Wieland T., Heineke J. (2024)

Endothelial derived, secreted long non-coding RNAs Gadlor1 and Gadlor2 aggravate cardiac remodeling

Mol.Ther.Nucleic Acids, 35 (3)

DOI: 10.1016/j.omtn.2024.102306 HZI-Repositorium PubMed

Gödecke N., Hauser H., Wirth D. (2024)

Stable Expression by Lentiviral Transduction of Cells

Methods Mol Biol, 2. edition (10)

DOI: 10.1007/978-1-0716-3878-1_10 HZI-Repositorium PubMed

Morales A.E., Gumenick R., Genovese C.M., Jang Y.Y., Ouedraogo A., Garayo M.I., Pannellini T., Patel S., Bott M.E., Alvarez J., Mun S.S., Totonchy J., Gautam A., La Mora J.D., Chang S., Wirth D., Horenstein M., Dao T., Scheinberg D.A., Rubinstein P.G., Semeere A., Martin J., Godfrey C.C., Moser C.B., Matining R.M., Campbell T.B., Borok M.Z., ... , Krown S.E., Cesarman E. (2024)

Wilms' tumor 1 (WT1) antigen is overexpressed in Kaposi Sarcoma and is regulated by KSHV vFLIP

PLoS Pathogens, 20 (1)

DOI: 10.1371/journal.ppat.1011881 HZI-Repositorium PubMed

Kutle I., Dittrich A., Wirth D. (2023)

Mouse Models for Human Herpesviruses

Pathogens, 12 (7)

DOI: 10.3390/pathogens12070953 HZI-Repositorium PubMed

Gödecke N., Herrmann S., Weichelt V., Wirth D. (2023)

A Ubiquitous Chromatin Opening Element and DNA Demethylation Facilitate Doxycycline-Controlled Expression during Differentiation and in Transgenic Mice

ACS Synth Biol, 12 (2)

DOI: 10.1021/acssynbio.2c00450 HZI-Repositorium PubMed

Latorre Y., Torres M., Vergara M., Berrios J., Sampayo M.M., Gödecke N., Wirth D., Hauser H., ... , Dickson A.J., Altamirano C. (2023)

Engineering of Chinese hamster ovary cells for co-overexpressing MYC and XBP1s increased cell proliferation and recombinant EPO production

Sci.Rep., 13 (1)

DOI: 10.1038/s41598-023-28622-z HZI-Repositorium PubMed

Schwerk J., Kemper L., Bussey K.A., Lienenklaus S., Weiss S., Cicin-Sain L., Kröger A., Kalinke U., Collins C.M., Speck S.H., Messerle M., Wirth D., Brinkmann M.M., ... , Hauser H., Köster M. (2022)

Type I Interferon Signaling Controls Gammaherpesvirus Latency In Vivo

Pathogens, 11 (12)

DOI: 10.3390/pathogens11121554 HZI-Repositorium PubMed

Lee M.J., Lee J., Kang S.K., Wirth D., Yoo S.M., ... , Park C., Lee M.S. (2022)

CXCL1 confers a survival advantage in Kaposi's sarcoma-associated herpesvirus-infected human endothelial cells through STAT3 phosphorylation

J.Med.Virol.

DOI: 10.1002/jmv.28020 PubMed

Gaur P., Riehn M., Zha L., Köster M., Hauser H., Wirth D. (2021)

Defective interferon amplification and impaired host responses against influenza virus in obese mice

Obesity.(Silver.Spring), 29 (8)

DOI: 10.1002/oby.23196 HZI-Repositorium PubMed

Rahman S.K., Ansari M.A., Gaur P., Ahmad I., Chakravarty C., Verma D.K., Sharma A., Chhibber S., Nehal N., ... , Wirth D., Lal S.K. (2021)

The Immunomodulatory CEA Cell Adhesion Molecule 6 (CEACAM6/CD66c) Is a Protein Receptor for the Influenza a Virus

Viruses., 13 (5)

DOI: 10.3390/v13050726 HZI-Repositorium PubMed

Kumashie K.G., Cebula M., Hagedorn C., Kreppel F., Pils M.C., Koch-Nolte F., ... , Rissiek B., Wirth D. (2021)

Improved Functionality of Exhausted Intrahepatic CXCR5+ CD8+ T Cells Contributes to Chronic Antigen Clearance Upon Immunomodulation

Front Immunol., 11

DOI: 10.3389/fimmu.2020.592328 HZI-Repositorium PubMed

Dubich T., Dittrich A., Bousset K., Geffers R., Büsche G., Köster M., Hauser H., ... , Schulz T.F., Wirth D. (2021)

3D culture conditions support Kaposi's sarcoma herpesvirus (KSHV) maintenance and viral spread in endothelial cells

J.Mol.Med.(Berl), 99 (3)

DOI: 10.1007/s00109-020-02020-8 HZI-Repositorium PubMed

Gödecke N., Herrmann S., Hauser H., Mayer-Bartschmid A., Trautwein M., Wirth D. (2021)

Rational Design of Single Copy Expression Cassettes in Defined Chromosomal Sites Overcomes Intraclonal Cell-to-Cell Expression Heterogeneity and Ensures Robust Antibody Production

ACS Synth.Biol., 10 (1)

DOI: 10.1021/acssynbio.0c00519 PubMed

Gödecke N., Riedel J., Herrmann S., Behme S., Rand U., Kubsch T., Cicin-Sain L., Hauser H., ... , Köster M., Wirth D. (2020)

Synthetic rewiring and boosting type I interferon responses for visualization and counteracting viral infections

Nucleic Acids Res., 48 (20)

DOI: 10.1093/nar/gkaa961 HZI-Repositorium PubMed

Brown R.J.P., Tegtmeyer B., Sheldon J., Khera T., Anggakusuma, Todt D., Vieyres G., Weller R., Joecks S., Zhang Y., Sake S., Bankwitz D., Welsch K., Ginkel C., Engelmann M., Gerold G., Steinmann E., Yuan Q., Ott M., Vondran F.W.R., Krey T., Ströh L.J., Miskey C., Ivics Z., Herder V., Baumgärtner W., Lauber C., Seifert M., Tarr A.W., Mcclure C.P., Randall G., Baktash Y., Ploss A., Thi V.L.D., Michailidis E., Saeed M., Verhoye L., Meuleman P., Goedecke N., Wirth D., ... , Rice C.M., Pietschmann T. (2020)

Liver-expressed Cd302 and Cr1l limit hepatitis C virus cross-species transmission to mice

Sci Adv, 6 (45)

DOI: 10.1126/sciadv.abd3233 HZI-Repositorium PubMed

Wahlicht T., Vieyres G., Bruns S.A., Meumann N., Büning H., Hauser H., Schmitz I., ... , Pietschmann T., Wirth D. (2020)

Controlled Functional Zonation of Hepatocytes in Vitro by Engineering of Wnt Signaling

ACS Synth.Biol., 9 (7)

DOI: 10.1021/acssynbio.9b00435 PubMed

Beauclair G., Naimo E., Dubich T., Rückert J., Koch S., Dhingra A., ... , Wirth D., Schulz T.F. (2020)

Targeting Kaposi's Sarcoma-Associated Herpesvirus ORF21 Tyrosine Kinase and Viral Lytic Reactivation by Tyrosine Kinase Inhibitors Approved for Clinical Use

J.Virol., 94 (5)

DOI: 10.1128/JVI.01791-19 HZI-Repositorium PubMed

Aghajanian H., Kimura T., Rurik J.G., Hancock A.S., Leibowitz M.S., Li L., Scholler J., Monslow J., Lo A., Han W., Wang T., Bedi K., Morley M.P., Saldana R.A.L., Bolar N.A., McDaid K., Assenmacher C.A., Smith C.L., Wirth D., June C.H., Margulies K.B., ... , Jain R., Albelda S.M. (2019)

Author Correction: Targeting cardiac fibrosis with engineered T cells (Nature, (2019), 573, 7774, (430-433), 10.1038/s41586-019-1546-z)

Nature, 576 (7785)

DOI: 10.1038/s41586-019-1761-7 PubMed

Aghajanian H., Kimura T., Rurik J.G., Hancock A.S., Leibowitz M.S., Li L., Scholler J., Monslow J., Lo A., Han W., Wang T., Bedi K., Morley M.P., Linares Saldana R.A., Bolar N.A., McDaid K., Assenmacher C.A., Smith C.L., Wirth D., June C.H., Margulies K.B., Jain R., Puré E., ... , Albelda S.M., Epstein J.A. (2019)

Targeting cardiac fibrosis with engineered T cells

Nature, 573 (7774)

DOI: 10.1038/s41586-019-1546-z HZI-Repositorium PubMed

Laske T., Bachmann M., Dostert M., Karlas A., Wirth D., Frensing T., Meyer T.F., ... , Hauser H., Reichl U. (2019)

Model-based analysis of influenza A virus replication in genetically engineered cell lines elucidates the impact of host cell factors on key kinetic parameters of virus growth

PLoS Comput.Biol., 15 (4)

DOI: 10.1371/journal.pcbi.1006944 HZI-Repositorium PubMed

Dubich T., Lieske A., Santag S., Beauclair G., Rückert J., Herrmann J., Gorges J., Büsche G., Kazmaier U., Hauser H., Stadler M., ... , Schulz T.F., Wirth D. (2019)

An endothelial cell line infected by Kaposi's sarcoma-associated herpes virus (KSHV) allows the investigation of Kaposi's sarcoma and the validation of novel viral inhibitors in vitro and in vivo

Journal of Molecular Medicine, 97 (3)

DOI: 10.1007/s00109-018-01733-1 HZI-Repositorium PubMed

Ullah S., Seidel K., Türkkan S., Warwas D.P., Dubich T., Rohde M., Hauser H., Behrens P., Kirschning A., ... , Köster M., Wirth D. (2019)

Macrophage entrapped silica coated superparamagnetic iron oxide particles for controlled drug release in a 3D cancer model

J.Control Release, 294

DOI: 10.1016/j.jconrel.2018.12.040 HZI-Repositorium PubMed

Becker J., Kinast V., Döring M., Lipps C., Duran V., Spanier J., Tegtmeyer P.K., Wirth D., Cicin-Sain L., ... , Alcami A., Kalinke U. (2018)

Human monocyte-derived macrophages inhibit HCMV spread independent of classical antiviral cytokines

Virulence., 9 (1)

DOI: 10.1080/21505594.2018.1535785 HZI-Repositorium

Tolle C., Riedel J., Mikolai C., Winkel A., Stiesch M., ... , Wirth D., Menzel H. (2018)

Biocompatible Coatings from Smart Biopolymer Nanoparticles for Enzymatically Induced Drug Release

Biomolecules, 8 (4)

DOI: 10.3390/biom8040103 PubMed

Gödecke N., Hauser H., Wirth D. (2018)

Stable Expression by Lentiviral Transduction of Cells

Methods Mol.Biol., 1850 (chapter 4)

DOI: 10.1007/978-1-4939-8730-6_4

Ziegler P.K., Bollrath J., Pallangyo C.K., Matsutani T., Canli Í., De Oliveira T., Diamanti M.A., Müller N., Gamrekelashvili J., Putoczki T., Horst D., Mankan A.K., Öner M.G., Müller S., Müller-Höcker J., Kirchner T., Slotta-Huspenina J., Taketo M.M., Reinheckel T., Dröse S., Larner A.C., Wels W.S., Ernst M., Greten T.F., Arkan M.C., Korn T., ... , Wirth D., Greten F.R. (2018)

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis

Cell, 174 (1)

DOI: 10.1016/j.cell.2018.05.028 HZI-Repositorium PubMed

Sapich S., Hittinger M., Hendrix-Jastrzebski R., Repnik U., Griffiths G., May T., Wirth D., Bals R., ... , Schneider-Daum N., Lehr C.M. (2018)

Murine alveolar epithelial cells and their lentivirus-mediated immortalisation

Altern.Lab Anim, 46 (2)

DOI: - PubMed

Lipps C., Klein F., Wahlicht T., Seiffert V., Butueva M., Zauers J., Truschel T., Luckner M., Köster M., MacLeod R., Pezoldt J., Hühn J., Yuan Q., Muller P.P., Kempf H., Zweigerdt R., Dittrich-Breiholz O., Pufe T., Beckmann R., Drescher W., Riancho J., Sanudo C., Korff T., Opalka B., Rebmann V., Gothert J.R., Alves P.M., Ott M., Schucht R., Hauser H., ... , Wirth D., May T. (2018)

Expansion of functional personalized cells with specific transgene combinations

Nat.Commun., 9 (1)

DOI: 10.1038/s41467-018-03408-4 HZI-Repositorium PubMed

Pieters T., Haenebalcke L., Bruneel K., Vandamme N., Hochepied T., Van Hengel J., Wirth D., Berx G., Haigh J.J., ... , van Roy F., Goossens S. (2017)

Structure-function studies in mouse embryonic stem cells using recombinase-mediated cassette exchange

J.Visualized Exp., 2017 (122)

DOI: 10.3791/55575 PubMed

Zhao G., Wirth D., Schmitz I., Meyer-Hermann M. (2017)

A mathematical model of the impact of insulin secretion dynamics on selective hepatic insulin resistance

Nat.Commun., 8 (1)

DOI: 10.1038/s41467-017-01627-9 HZI-Repositorium PubMed

Selvakumar T.A., Bhushal S., Kalinke U., Wirth D., Hauser H., ... , Köster M., Hornef M.W. (2017)

Identification of a Predominantly Interferon-lambda-Induced Transcriptional Profile in Murine Intestinal Epithelial Cells

Front Immunol., 8

DOI: 10.3389/fimmu.2017.01302 HZI-Repositorium PubMed

Riehn M., Cebula M., Hauser H., Wirth D. (2017)

CpG-ODN Facilitates Effective Intratracheal Immunization and Recall of Memory against Neoantigen-Expressing Alveolar Cells

Front Immunol., 8

DOI: 10.3389/fimmu.2017.01201 HZI-Repositorium PubMed

Gödecke N., Zha L., Spencer S., Behme S., Riemer P., Rehli M., ... , Hauser H., Wirth D. (2017)

Controlled re-activation of epigenetically silenced Tet promoter-driven transgene expression by targeted demethylation

Nucleic Acids Res., 45 (16)

DOI: 10.1093/nar/gkx601 HZI-Repositorium

Pasztoi M., Pezoldt J., Beckstette M., Lipps C., Wirth D., Rohde M., Paloczi K., ... , Buzas E.I., Hühn J. (2017)

Mesenteric lymph node stromal cell-derived extracellular vesicles contribute to peripheral de novo induction of Foxp3+ regulatory T cells

Eur.J.Immunol., 47 (12)

DOI: 10.1002/eji.201746960 HZI-Repositorium PubMed

Kunkiel J., Godecke N., Ackermann M., Hoffmann D., Schambach A., Lachmann N., ... , Wirth D., Moritz T. (2017)

The CpG-sites of the CBX3 ubiquitous chromatin opening element are critical structural determinants for the anti-silencing function

Sci.Rep., 7 (1)

DOI: 10.1038/s41598-017-04212-8 HZI-Repositorium

Cebula M., Riehn M., Hillebrand U., Kratzer R.F., Kreppel F., Koutsoumpli G., Daemen T., ... , Hauser H., Wirth D. (2017)

TLR9-Mediated Conditioning of Liver Environment Is Essential for Successful Intrahepatic Immunotherapy and Effective Memory Recall

Mol.Ther.

DOI: 10.1016/j.ymthe.2017.06.018 HZI-Repositorium PubMed

Strandt H., Pinheiro D.F., Kaplan D.H., Wirth D., Gratz I.K., Hammerl P., ... , Thalhamer J., Stoecklinger A. (2017)

Neoantigen Expression in Steady-State Langerhans Cells Induces CTL Tolerance

J.Immunol., 199 (5)

DOI: 10.4049/jimmunol.1602098 HZI-Repositorium

Bhushal S., Wolfsmüller M., Selvakumar T.A., Kemper L., Wirth D., Hornef M.W., ... , Hauser H., Köster M. (2017)

Cell polarization and epigenetic status shape the heterogeneous response to type III interferons in intestinal epithelial cells

Front.Immunol., 8 (JUN)

DOI: 10.3389/fimmu.2017.00671 HZI-Repositorium PubMed

Schmidt M., Laufs S., Aiuti A., Aubourg P., Baum C., Biasco L., Cartier N., Hauser H., Montini E., Moullier P., Snyder R.O., ... , Wirth D., von Kalle C. (2012)

Biosafety analysis in preclinical and clinical studies

(B2-3)

DOI: - PubMed

Gama-Norton L., Hauser H., Wirth D. (2012)

Restrictions and requirements for stable lentiviral vector production in HEK293 cells

(A3-6)

DOI: - PubMed

Coroadinha A.S., Wirth D., Rodrigues A.F., Gama-Norton L., Duros C., Artus A., Cohen-Haguenauer O., Marques Alves P., Cruz P.E., ... , Carrondo M.J.T., Hauser H. (2012)

Modular retroviral producer cell lines

(A2-4)

DOI: - PubMed

Gaur P., Cebula M., Riehn M., Hillebrand U., Hauser H., Wirth D. (2017)

Diet induced obesity has an influence on intrahepatic T cell responses

Metab.Clin.Exp., 69

DOI: 10.1016/j.metabol.2017.01.022 HZI-Repositorium PubMed

Lipps C., Badar M., Butueva M., Dubich T., Singh V.V., Rau S., Weber A., Kracht M., Koster M., May T., Schulz T.F., ... , Hauser H., Wirth D. (2016)

Proliferation status defines functional properties of endothelial cells

Cell Mol.Life Sci, 74 (7)

DOI: 10.1007/s00018-016-2417-5 HZI-Repositorium PubMed

Pieters T., Goossens S., Haenebalcke L., Andries V., Stryjewska A., De Rycke R., Lemeire K., Hochepied T., Huylebroeck D., Berx G., Stemmler M.P., Wirth D., Haigh J.J., ... , van Hengel J., van Roy F. (2016)

p120 Catenin-Mediated Stabilization of E-Cadherin Is Essential for Primitive Endoderm Specification

PLoS Genet., 12 (8)

DOI: 10.1371/journal.pgen.1006243 HZI-Repositorium PubMed

Spencer S., Gugliotta A., Gödecke N., Hauser H., Wirth D. (2016)

Epigenetic modulations rendering cell-to-cell variability and phenotypic metastability

J Genet.Genomics, 43

DOI: 10.1016/j.jgg.2016.05.008 PubMed

Lipps C., May T., Wirth D. (2014)

Cell lines: Generation of cell lines and biotechnological applications

DOI: 10.1515/9783110278965.11

Köster M., Wirth D. (2016)

Zellen im Zeitraffer - Dynamik der Genexpression in Echtzeit [Cells in time lapse - Dynamics of gene expression in real time]

Biospektrum, 22 (1)

DOI: 10.1007/s12268-016-0657-4

Rais B., Köster M., Rahim M.I., Pils M., Seitz J.M., Hauser H., ... , Wirth D., Müller P.P. (2016)

Evaluation of the inflammatory potential of implant materials in a mouse model by bioluminescent imaging of intravenously injected bone marrow cells

J Biomed.Mater.Res A, 104 (9)

DOI: 10.1002/jbm.a.35758 HZI-Repositorium PubMed

Lipps C., May T., Wirth D. (2014)

Generation of cell lines and biotechnological applications

(2.1)

DOI: -

Kruse N., Spencer S., Wirth D. (2014)

Rational Approaches for Transgene Expression: Targeted Integration and Episomal Maintenance

Kuehn A., Kletting S., de Souza Carvalho-Wodarz, Repnik U., Griffiths G., Fischer U., Meese E., Huwer H., Wirth D., May T., ... , Schneider-Daum N., Lehr C.M. (2016)

Human alveolar epithelial cells expressing tight junctions to model the air-blood barrier

ALTEX., 33

DOI: 10.14573/altex.1511131 HZI-Repositorium PubMed

Lempp F.A., Mutz P., Lipps C., Wirth D., Bartenschlager R., Urban S. (2015)

Evidence that hepatitis B virus replication in mouse cells is limited by the lack of a host cell dependency factor

J Hepatol., 64

DOI: 10.1016/j.jhep.2015.10.030 PubMed

Bachmann M., Breitwieser T., Lipps C., Wirth D., Jordan I., ... , Reichl U., Frensing T. (2016)

Impaired antiviral response of adenovirus-transformed cell lines supports virus replication

J.Gen.Virol., 97 (2)

DOI: 10.1099/jgv.0.000361 HZI-Repositorium PubMed

Tran D.D.H., Koch A., Saran S., Armbrecht M., Ewald F., Koch M., Wahlicht T., Wirth D., Braun A., Nashan B., ... , Gaestel M., Tamura T. (2016)

Extracellular-signal regulated kinase (Erk1/2), mitogen-activated protein kinase-activated protein kinase 2 (MK2) and tristetraprolin (TTP) comprehensively regulate injury-induced immediate early gene (IEG) response in in vitro liver organ culture

Cell.Signal., 28 (5)

DOI: 10.1016/j.cellsig.2016.02.007 PubMed

Ochel A., Cebula M., Riehn M., Hillebrand U., Lipps C., Schirmbeck R., ... , Hauser H., Wirth D. (2016)

Effective intrahepatic CD8+ T-cell immune responses are induced by low but not high numbers of antigen-expressing hepatocytes

Cell Mol.Immunol., 13

DOI: 10.1038/cmi.2015.80 HZI-Repositorium PubMed

Lieber D., Hochdorfer D., Stoehr D., Schubert A., Lotfi R., May T., ... , Wirth D., Sinzger C. (2015)

A permanently growing human endothelial cell line supports productive infection with human cytomegalovirus under conditional cell growth arrest

Biotechniques, 59 (3)

Rand U., Riedel J., Hillebrand U., Shin D., Willenberg S., Behme S., Klawonn F., Köster M., ... , Hauser H., Wirth D. (2015)

Single-cell analysis reveals heterogeneity in onset of transgene expression from synthetic tetracycline-dependent promoters

Biotechnol.J., 10 (2)

Spencer S., Gugliotta A., Koenitzer J., Hauser Hansjörg, Wirth Dagmar (2014)

Stability of single copy transgene expression in CHOK1 cells is affected by histone modifications but not by DNA methylation

Journal of Biotechnology, 195C

Rand U., Hillebrand Upneet, Sievers S., Willenberg S., Köster Mario, ... , Hauser Hansjörg, Wirth Dagmar (2014)

Uncoupling of the dynamics of host-pathogen interaction uncovers new mechanisms of viral interferon antagonism at the single-cell level

Nucleic Acids Res, 42 (13)

DOI: 10.1093/nar/gku492 HZI-Repositorium PubMed

Dag F., Dolken L., Holzki J., Drabig A., Weingartner A., Schwerk J., Lienenklaus S., Conte I., Geffers R., Davenport C., Rand U., Köster M., Weiss S., Adler B., Wirth D., Messerle M., ... , Hauser H., Cicin-Sain L. (2014)

Reversible silencing of cytomegalovirus genomes by type I interferon governs virus latency

PLoS Pathogens, 10 (2)

DOI: 10.1371/journal.ppat.1003962 HZI-Repositorium PubMed

Botezatu L., Sievers S., Gama-Norton L., Schucht Roland, Hauser Hansjörg, Wirth Dagmar (2012)

Genetic aspects of cell line development from a synthetic biology perspective

Advances in Biochemical Engineering/Biotechnology, 127

Dag F., Weingartner A., Butueva M., Conte I., Holzki J., May T., Adler B., ... , Wirth D., In-Ain I. (2013)

A new reporter mouse cytomegalovirus reveals maintained immediate-early gene expression but poor virus replication in cycling liver sinusoidal endothelial cells

Virology Journal, 10 (1)

DOI: 10.1186/1743-422X-10-197 HZI-Repositorium PubMed

Lipps C., May T., Hauser H., Wirth D. (2013)

Eternity and functionality - rational access to physiologically relevant cell lines

Biological Chemistry, 394 (12)

PubMed

Cebula Marcin, Ochel Aaron, Hillebrand Upneet, Pils Marina C., Schirmbeck R., ... , Hauser Hansjörg, Wirth Dagmar (2013)

An Inducible Transgenic Mouse Model for Immune Mediated Hepatitis Showing Clearance of Antigen Expressing Hepatocytes by CD8+ T Cells

PLoS ONE, 8 (7)

DOI: 10.1371/journal.pone.0068720 HZI-Repositorium PubMed

Haenebalcke L., Goossens S., Naessens M., Kruse N., Ghahremani M.F., Bartunkova S., Haigh K., Pieters T., Dierickx P., Drogat B., Nyabi O., ... , Wirth Dagmar, Haigh J.J. (2013)

Efficient ROSA26-Based Conditional and/or Inducible Transgenesis Using RMCE-Compatible F1 Hybrid Mouse Embryonic Stem Cells

Stem Cell Reviews and Reports, 9

Haenebalcke L., Goossens S., Dierickx P., Bartunkova S., D'Hont J., Haigh K., Hochepied T., Wirth Dagmar, ... , Nagy A., Haigh J.J. (2013)

The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (De)differentiation

Cell Reports, 3 (2)

DOI: 10.1016/j.celrep.2013.01.016 HZI-Repositorium PubMed

May T., Hauser Hansjörg, Wirth Dagmar (2012)

Cell systems: Functional cell lines through new immortalization protocols [Zellsysteme: Funktionale zelllinien durch neue immortalisierungsprotokolle]

Biospektrum, 18 (4)

Nehlsen K., da Gama-Norton L., Schucht R., Hauser Hansjörg*, Wirth Dagmar* (2011)

Towards rational engineering of cells: Recombinant gene expression in defined chromosomal loci

BMC proceedings, 5 ((Suppl 8))

DOI: 10.1186/1753-6561-5-S8-O6 HZI-Repositorium PubMed

Cruz P.E., Rodrigues T., Carmo M., Wirth Dagmar*, Amaral A.I., ... , Alves P.M., Coroadinha A.S. (2011)

Manufacturing of retroviruses

Methods in Molecular Biology, 737

Wilke S., Gröbe Lothar*, Maffenbeier V., Jäger Volker*, Gossen M., Josewski J., Duda A., Polle L., Owens R.J., Wirth Dagmar*, Heinz Dirk* W., ... , van den Heuvel Joop*, Büssow Konrad* (2011)

Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development

PLoS ONE, 6 (12)

DOI: 10.1371/journal.pone.0027829 HZI-Repositorium PubMed

Gama-Norton L., Botezatu L., Herrmann S., Schweizer M., Alves P.M., ... , Hauser Hansjörg*, Wirth Dagmar* (2011)

Lentivirus production is influenced by SV40 large T-antigen and chromosomal integration of the vector in HEK293 cells

Human gene therapy, 22 (10)

DOI: 10.1089/hum.2010.143 HZI-Repositorium PubMed

Alkharsah K.R., Singh V.V., Bosco R., Santag S., Grundhoff A., Konrad A., Sturzl M., Wirth Dagmar*, Dittrich-Breiholz O., ... , Kracht M., Schulz T.F. (2011)

Deletion of Kaposi's sarcoma-associated herpesvirus FLICE inhibitory protein, vFLIP, from the viral genome compromises the activation of STAT1-responsive cellular genes and spindle cell formation in endothelial cells

Journal of Virology, 85 (19)

Schucht R., Lydford S., Andzinski L., Zauers J., Cooper J., Hauser Hansjörg*, ... , Wirth Dagmar*, May T. (2011)

Rapid establishment of G-protein-coupled receptor-expressing cell lines by site-specific integration

Journal of Biomolecular Screening, 16 (3)

DOI: 10.1177/1087057110396371 HZI-Repositorium PubMed

Sandhu U., Cebula M., Behme S., Riemer P., Wodarczyk C., Metzger D., Reimann J., Schirmbeck R., ... , Hauser Hansjörg*, Wirth Dagmar* (2011)

Strict control of transgene expression in a mouse model for sensitive biological applications based on RMCE compatible ES cells

Nucleic Acids Research, 39 (1)

Carrondo M., Panet M., Wirth Dagmar*, Coroadinha A.S., Cruz P., Falk H., Schucht R., Dupont F., Geny-Fiamma C., ... , Merten O.W., Hauser Hansjörg* (2011)

Integrated strategy for the production of therapeutic retroviral vectors

Human gene therapy, 22 (3)

DOI: 10.1089/hum.2009.165 HZI-Repositorium PubMed

Schucht R., Wirth Dagmar*, May T. (2010)

Precise regulation of transgene expression level and control of cell physiology

Cell Biology and Toxicology, 26 (1)

Loew R., Meyer Y., Kuehlcke K., Gama-Norton L., Wirth D., Hauser H., Stein S., Grez M., Thornhill S., Thrasher A., ... , Baum C., Schambach A. (2010)

A new PG13-based packaging cell line for stable production of clinical-grade self-inactivating gamma-retroviral vectors using targeted integration

Gene Therapy, 17 (2)

DOI: 10.1038/gt.2009.134 PubMed

May T., Butueva M., Bantner S., Markusic D., Seppen J., MacLeod R.A., Weich H., ... , Hauser Hansjörg*, Wirth Dagmar* (2010)

Synthetic gene regulation circuits for control of cell expansion

Tissue Engineering - Part A, 16 (2)

Gama-Norton L., Herrmann S., Schucht R., Coroadinha A.S., Low R., Alves P.M., Bartholomae C.C., Schmidt M., Baum C., Schambach A., ... , Hauser Hansjörg*, Wirth Dagmar* (2010)

Retroviral vector performance in defined chromosomal Loci of modular packaging cell lines

Human gene therapy, 21 (8)

DOI: 10.1089/hum.2009.089 HZI-Repositorium PubMed

Wirth Dagmar*, Gama-Norton L., Schucht R., Nehlsen K., Hauser Hansjörg* (2009)

Site-directed engineering of defined chromosomal sites for recombinant protein and virus expression

BioPharm International, 22 (7)

Nehlsen K., Herrmann S., Zauers J, Hauser Hansjörg*, Wirth Dagmar* (2010)

Toxin-antitoxin based transgene expression in mammalian cells.

Nucleic Acids Research, 38 (5)

DOI: 10.1093/nar/gkp1140 HZI-Repositorium PubMed

Nehlsen K., Schucht R., da Gama-Norton L., Kromer W., Baer A., Cayli A., ... , Hauser Hansjörg*, Wirth Dagmar* (2009)

Recombinant protein expression by targeting pre-selected chromosomal loci

BMC Biotechnology, 14 (9)

DOI: 10.1186/1472-6750-9-100 HZI-Repositorium

May T., Hauser Hansjörg*, Wirth Dagmar* (2007)

In vitro expansion of tissue cells by conditional proliferation

Methods in Molecular Medicine, 140

May T., Eccleston L., Herrmann S., Hauser Hansjörg*, Goncalves J., Wirth Dagmar* (2008)

Bimodal and hysteretic expression in mammalian cells from a synthetic gene circuit

PLoS ONE, 3 (6)

DOI: 10.1371/journal.pone.0002372 HZI-Repositorium PubMed

Wirth Dagmar*, Gama-Norton L., Riemer P., Sandhu U., Schucht R., Hauser Hans-Jörg* (2007)

Road to precision: recombinase-based targeting technologies for genome engineering

Current Opinion in Biotechnology, 18 (5)

May Tobias*, Hauser Hans-Jörg*, Wirth Dagmar* (2007)

In vitro expansion of tissue cells by conditional proliferation

Kues W.A., Schwinzer R., Wirth Dagmar*, Verhoeyen E., Lemme E., Herrmann D., Barg-Kues B., Hauser Hansjörg*, ... , Wonigeit K., Niemann H. (2006)

Reactivation of silenced tetracycline-controlled hRCA constructs in transgenic pigs

Xenotransplantation, 13 (6)

May T., Hauser Hansjörg*, Wirth Dagmar* (2006)

Current status of transcriptional regulation systems

Cytotechnology, 50

Kues W.A., Schwinzer R., Wirth Dagmar*, Verhoeyen E., Lemme E., Herrmann D., Barg-Kues B., Hauser Hansjörg*, ... , Wonigkeit K., Niemann H. (2006)

Epigenetic silencing and tissue independent expression of a novel tetracycline inducible system in double transgenic pigs

FASEB journal, 20

Coroadinha A.S., Schucht R., Gama-Norton L., Wirth Dagmar*, Hauser Hansjörg*, Carrondo M.J.T. (2006)

The use of recombinase cassette exchange in retroviral vector producer cell lines: predictability and efficiency in transgene replacement

Journal of Biotechnology, 124 (2)

DOI: 10.1016/j.jbiotec.2006.01.037 HZI-Repositorium

Deppenmeier S., Bock O., Mengel M., Niemann H., Kues W., Lemme E., Wirth Dagmar*, ... , Wonigeit K., Kreipe H. (2006)

Health status of transgenic pigs expressing the human complement regulatory protein CD59

Xenotransplantation, 13 (4)

DOI: 10.1111/j.1399-3089.2006.00317.x HZI-Repositorium

Schucht R., Coroadinha A.S., Zanta-Boussif M.A., Verhoeyen E., Carrondo M.J.T., ... , Hauser Hansjörg*, Wirth Dagmar* (2006)

A new generation of retroviral producer cells: Predictable and stable virus production by Flp mediated site-specific integration of retroviral vectors

Molecular therapy, 14

DOI: 10.1016/j.ymthe.2005.12.003 HZI-Repositorium PubMed

Thiefes A., Wolter S., Mushinski J.S., Hoffmann E., Dittrich-Breiholz O., Graue N., Dörrie A., Schneider H., Wirth D., Luckow B., ... , Resch K., Kracht M. (2005)

Simultaneous blockade of NFkappaB, JNK and p38 MAPK by a kinase-inactive mutant of the protein kinase TAK1 sensitizes cells to apoptosis and affects a distinct spectrum of TNF target genes

Journal of Biological Chemistry, 280

DOI: 10.1074/jbc.M411657200 HZI-Repositorium PubMed

May T., Müller P., Weich H., Froese N., Deutsch U., Wirth D., ... , Kröger A., Hauser H. (2005)

Establishment of murine cell lines by constitutive and conditional immortalization

Journal of Biotechnology, 120 (1)

May T., Wirth D., Hauser H., Müller P.P. (2005)

Transcriptionally regulated immortalization overcomes side effects of tmeperature-sensitive SV40 large T antigen

Biochemical and Biophysical Research Communications, 327 (3)

May T., Lindenmaier W., Wirth D., Müller P.P. (2004)

Application of a reversible immortalisation system for the generation of proliferation-controlled cell lines

Cytotechnology, 46

Weissenbach M., Clahsen T., Weber C., Spitzer D., Wirth D., Vestwever D., ... , Heinrich P.C., Schaper F. (2004)

Interleukin-6 is a direct mediator of T cell migration

European Journal of Immunology, 34

Unsinger J., Lindenmaier W., May T., Hauser H., Wirth D. (2004)

Stable and strictly controlled expression of LTR-flanked autoregulated expression cassettes upon adenoviral transfer

Biochemical and Biophysical Research Communications, 319

May T., Hauser H., Wirth D. (2004)

Transcriptional control of SV40 expression allows a complete reversion of immortalization

Nucleic Acids Research, 32

Wirth Dagmar, Hauser Hansjörg (2004)

Flp-mediated integration of expression cassettes into FRT tagged chromosomal loci

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Müller P.P., Wirth D., Unsinger U., Hauser H. (2003)

Genetic approaches to recombinant protein production in mammalian cells

Spitzer D., Dittmar K.E., Rohde M., Hauser H., Wirth D. (2003)

Green fluorescent protein-tagged retroviral envelope protein for analysis of virus-cell interactions.

Journal of Virology, 77

Hirschmann F., Verhoeyen E., Wirth D., Bauwens S., Hauser H., Rudert M. (2002)

Vital marking of articular chondrocytes by retroviral infection using green fluorescence protein

Osteoarthritis and Cartilage, 10 (2)

Verhoeyen E., Hauser H., Wirth D. (2001)

Evaluation of retroviral vector design in defined chromosomal loci by Flp-mediated cassette replacement

Human gene therapy, 12 (8)

Unsinger J., Kroger A., Hauser H., Wirth D. (2001)

Retroviral vectors for the transduction of autoregulated, bidirectional expression cassettes

Molecular therapy, 4 (5)

Niemann H., Verhoeyen E., Wonigeit K., Lorenz R., Hecker J., Schwinzer R., Hauser H., Kues W.A., Halter R., Lemme E., Herrmann D., Winkler M., ... , Wirth D., Paul D. (2001)

Cytomegalovirus early promoter induced expression of hCD59 in porcine organs provides protection against hyperacute rejection

Transplantation, 72 (12)

Müller P., Oumard A., Wirth D., Kröger A., Hauser H. (2001)

Polyvalent vectors for coexpression of multiple genes

Hauser H., Spitzer D., Verhoeyen E., Unsinger J., Wirth D. (2000)

New approaches towards ex vivo and in vivo gene therapy

Cells Tissues Organs, 167 (2-3)

Prof. Dr. Dagmar Wirth

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